Mouse Monoclonal p16 antibody [2D9A12] (STJ98295)

$379.00

Reactivity: Human, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal p16 antibody [2D9A12] (STJ98295)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CDKN2A antibody, CDKN2 antibody, MLM antibody,||
SCBT cat No: To be updated

 

Name

p16 Monoclonal Antibody

Catalogue No.

STJ98295

Reactivity

Human, Rat

Specificity

p16 Monoclonal Antibody detects endogenous levels of p16 protein.

Immunogen

Purified recombinant fragment of p16 expressed in E Coli

Host

Mouse

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG2b

Formulation

p16 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

p16 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tumor suppressor ARF antibody, Alternative reading frame antibody, ARF antibody, Cyclin-dependent kinase inhibitor 2A antibody, p14ARF antibody

Database links

Human UniProt/Swiss-Prot:Q8N726;Mouse UniPort/Swiss-Prot: P51480;Rat UniProt/Swiss-Port: Q9R0Z3;Human Entrez Gene: 1029;Mouse Entrez Gene: 12578;Rat Entrez Gene: Rn.48717

Protein names

Tumor suppressor ARF , Alternative reading frame , ARF , Cyclin-dependent kinase inhibitor 2A , p14ARF

Protein function

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its ‘Lys63’-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform smARF may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.

Protein post-translational modifications

Ubiquitinated in normal cells by TRIP12 via the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination at the N-terminus, regardeless of the absence of lysine residues. Ubiquitination leads to its degradation. In cancer cells, however, TRIP12 is located in a different cell compartment, preventing ubiquitination and degradation.

Protein cellular localization

Nucleus > nucleolus / Nucleus > nucleoplasm / Mitochondrion

Research area

All research areas>Cell Cycle Proteins>p15
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize p16 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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