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Mouse Monoclonal PAKgamma antibody [3B5] (STJ98304)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
PAK2 antibody,|C-t-PAK2 antibody|CB422 antibody|EC 22.214.171.124 antibody|Gamma PAK antibody|Gamma-PAK antibody|hPAK65 antibody|Kinase antibody|p21 (CDKN1A) activated kinase 2 antibody|p21 (CDKN1A)-activated kinase 2a antibody|p21 activated kinase 2 antibody|p21 protein (Cdc42/Rac)-activated kinase 2 antibody|p21 protein Cdc42 Rac activated kinase 2 antibody|p21-activated kinase 2 antibody|p21-activated kinase, 65-KD antibody|p21-activated protein kinase I antibody|p21CDKN1A activated kinase 2 antibody|p27 antibody|p34 antibody|p58 antibody|p65PAK antibody|PAK 2 antibody|PAK-2 antibody|PAK-2p34 antibody|Pak2 antibody|PAK2_HUMAN antibody|PAK65 antibody|PAKgamma antibody|S6 H4 kinase antibody|S6/H4 kinase antibody|Serine threonine protein kinase PAK 2 antibody|Serine/threonine protein kinase PAK 2 antibody|Anti-PAK2 antibody [EP796Y] (ab76293)
SCBT cat No: sc-48826|sc-10717|sc-376010|sc-67321|sc-54758|sc-136385|sc-271066|sc-13991|sc-48349|sc-69699|sc-514908|sc-7012|sc-32281|
PAKgamma Monoclonal Antibody
|Catalogue No.|| |
PAKgamma Monoclonal Antibody detects endogenous levels of PAKgamma protein.
Purified recombinant fragment of PAKgamma expressed in E Coli
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000
PAKgamma Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
PAKgamma Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Serine/threonine-protein kinase PAK 2 antibody, Gamma-PAK antibody, PAK65 antibody, S6/H4 kinase antibody, p21-activated kinase 2 antibody, PAK-2 antibody, p58 antibody
|Protein names|| |
Serine/threonine-protein kinase PAK 2 , Gamma-PAK , PAK65 , S6/H4 kinase , p21-activated kinase 2 , PAK-2 , p58
|Protein function|| |
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation. / ATP + a protein = ADP + a phosphoprotein. / Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage, autophosphorylated PAK-2p34 is constitutively active.
|Protein tissue specificity|| |
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. / Contains 1 CRIB domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Full length PAK2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate. / During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34. / Ubiquitinated, leading to its proteasomal degradation. / PAK-2p34 is myristoylated.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Cytoplasm > perinuclear region / Membrane; Lipid-anchor
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St John’s Laboratory Ltd.
|Product type|| |
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