Mouse Monoclonal PAKgamma antibody [3B5] (STJ98304)

$379.00

Reactivity: Human, Monkey
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal PAKgamma antibody [3B5] (STJ98304)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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PAK2 antibody,|C-t-PAK2 antibody|CB422 antibody|EC 2.7.11.1 antibody|Gamma PAK antibody|Gamma-PAK antibody|hPAK65 antibody|Kinase antibody|p21 (CDKN1A) activated kinase 2 antibody|p21 (CDKN1A)-activated kinase 2a antibody|p21 activated kinase 2 antibody|p21 protein (Cdc42/Rac)-activated kinase 2 antibody|p21 protein Cdc42 Rac activated kinase 2 antibody|p21-activated kinase 2 antibody|p21-activated kinase, 65-KD antibody|p21-activated protein kinase I antibody|p21CDKN1A activated kinase 2 antibody|p27 antibody|p34 antibody|p58 antibody|p65PAK antibody|PAK 2 antibody|PAK-2 antibody|PAK-2p34 antibody|Pak2 antibody|PAK2_HUMAN antibody|PAK65 antibody|PAKgamma antibody|S6 H4 kinase antibody|S6/H4 kinase antibody|Serine threonine protein kinase PAK 2 antibody|Serine/threonine protein kinase PAK 2 antibody|Anti-PAK2 antibody [EP796Y] (ab76293)
SCBT cat No: sc-48826|sc-10717|sc-376010|sc-67321|sc-54758|sc-136385|sc-271066|sc-13991|sc-48349|sc-69699|sc-514908|sc-7012|sc-32281|

 

Name

PAKgamma Monoclonal Antibody

Catalogue No.

STJ98304

Reactivity

Human, Monkey

Specificity

PAKgamma Monoclonal Antibody detects endogenous levels of PAKgamma protein.

Immunogen

Purified recombinant fragment of PAKgamma expressed in E Coli

Host

Mouse

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

PAKgamma Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

PAKgamma Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Serine/threonine-protein kinase PAK 2 antibody, Gamma-PAK antibody, PAK65 antibody, S6/H4 kinase antibody, p21-activated kinase 2 antibody, PAK-2 antibody, p58 antibody

Database links

Human UniProt/Swiss-Prot:Q13177;Mouse UniPort/Swiss-Prot: Q8CIN4;Rat UniProt/Swiss-Port: Q64303;Human Entrez Gene: 5062;Mouse Entrez Gene: 224105;Rat Entrez Gene: Rn.3840

Protein names

Serine/threonine-protein kinase PAK 2 , Gamma-PAK , PAK65 , S6/H4 kinase , p21-activated kinase 2 , PAK-2 , p58

Protein function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation. / ATP + a protein = ADP + a phosphoprotein. / Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage, autophosphorylated PAK-2p34 is constitutively active.

Protein tissue specificity

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

Protein sequence and domain

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. / Contains 1 CRIB domain. / Contains 1 protein kinase domain.

Protein post-translational modifications

Full length PAK2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate. / During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34. / Ubiquitinated, leading to its proteasomal degradation. / PAK-2p34 is myristoylated.

Protein cellular localization

Cytoplasm / Nucleus / Cytoplasm > perinuclear region / Membrane; Lipid-anchor

Research area

All research areas>Kinases and Phosphatases>PAK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize PAKgamma Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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