Mouse Monoclonal PDK1 antibody [4A11] (STJ98315)

$379.00

Reactivity: Human, Rat, Monkey
Applications: WB, IHC, IF, FC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal PDK1 antibody [4A11] (STJ98315)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, FC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for PDK1 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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PDK1 antibody, PDHK1 antibody,|[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody|HGNC:8809 antibody|Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody|PDH kinase 1 antibody|Pdk1 antibody|PDK1_HUMAN antibody|Pyruvate dehydrogenase kinase isoform 1 antibody|Pyruvate dehydrogenase kinase, isoenzyme 1 antibody|Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] (ab110025)
SCBT cat No: sc-28783|sc-293160|sc-7140|sc-30691|

 

Name

PDK1 Monoclonal Antibody

Catalogue No.

STJ98315

Reactivity

Human, Rat, Monkey

Specificity

PDK1 Monoclonal Antibody detects endogenous levels of PDK1 protein.

Immunogen

Purified recombinant fragment of human PDK1 expressed in E Coli

Host

Mouse

Applications

WB, IHC, IF, FC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

PDK1 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

PDK1 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

kinase isozyme 1, mitochondrial antibody, Pyruvate dehydrogenase kinase isoform 1 antibody, PDH kinase 1 antibody

Database links

Human UniProt/Swiss-Prot:Q15118;Mouse UniPort/Swiss-Prot: Q8BFP9;Rat UniProt/Swiss-Port: Q5FVT5;Human Entrez Gene: 5163;Mouse Entrez Gene: 228026;Rat Entrez Gene: Rn.11185

Protein names

kinase isozyme 1, mitochondrial , Pyruvate dehydrogenase kinase isoform 1 , PDH kinase 1

Protein function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. / ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate. / Activity is enhanced by binding to the pyruvate dehydrogenase subunit DLAT. Inhibited by AZD7545; this compound interferes with DLAT binding and thereby inhibits kinase activity. Inhibited by dichloroacetate and radicicol.

Protein tissue specificity

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

Protein sequence and domain

Belongs to the PDK/BCKDK protein kinase family. / Contains 1 histidine kinase domain.

Protein post-translational modifications

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.

Protein cellular localization

Mitochondrion matrix

Research area

All research areas>Synthesis and Degradation>PDK1
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize PDK1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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