Mouse Monoclonal PERK antibody [5G5] (STJ98319)


Reactivity: Human
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal PERK antibody [5G5] (STJ98319)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody


EIF2AK3 antibody, PEK antibody, PERK antibody,|DKFZp781H1925 antibody|E2AK3_HUMAN antibody|EC antibody|Eif2ak3 antibody|Eukaryotic translation initiation factor 2 alpha kinase 3 antibody|Eukaryotic translation initiation factor 2-alpha kinase 3 antibody|Heme regulated EIF2 alpha kinase antibody|HRI antibody|HsPEK antibody|Pancreatic eIF2 alpha kinase antibody|Pancreatic eIF2-alpha kinase antibody|PEK antibody|PRKR like endoplasmic reticulum kinase antibody|PRKR-like endoplasmic reticulum kinase antibody|WRS antibody|Anti-PERK antibody (ab65142)
SCBT cat No: sc-377400|sc-9481|sc-7383|sc-13073|sc-9476|



PERK Monoclonal Antibody

Catalogue No.





PERK Monoclonal Antibody detects endogenous levels of PERK protein.


Purified recombinant fragment of human PERK expressed in E Coli





Recommended dilution

WB 1:500-1:2000; ELISA 1:10000








PERK Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.


1 mg/ml


PERK Antibody was purified using affinity purification.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Eukaryotic translation initiation factor 2-alpha kinase 3 antibody, PRKR-like endoplasmic reticulum kinase antibody, Pancreatic eIF2-alpha kinase antibody, HsPEK antibody

Database links

Human UniProt/Swiss-Prot:Q9NZJ5;Mouse UniPort/Swiss-Prot: Q9Z2B5;Rat UniProt/Swiss-Port: Q9Z1Z1;Human Entrez Gene: 9451;Mouse Entrez Gene: 13666;Rat Entrez Gene: Rn.24897

Protein names

Eukaryotic translation initiation factor 2-alpha kinase 3 , PRKR-like endoplasmic reticulum kinase , Pancreatic eIF2-alpha kinase , HsPEK

Protein function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on ‘Ser-52’ during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. / ATP + a protein = ADP + a phosphoprotein. / Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction.

Protein tissue specificity

Ubiquitous. A high level expression is seen in secretory tissues.

Involvement in disease

Wolcott-Rallison syndrome (WRS) [MIM:226980]: A rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. / Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop (By similarity). Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity. / N-glycosylated. / ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity.

Protein cellular localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Research area

All research areas>Kinases and Phosphatases>PERK
(View all antibody categories related to Kinases and Phosphatases)


AntibodyPlus can customize PERK Antibody according to your requirement, including bulk product size,etc. Please contact AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


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