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Mouse Monoclonal PHF8 antibody (STJ98531)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB
Recommended dilution: WB 1:1000-1:2000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
PHF8 antibody, KIAA1111 antibody, ZNF422 antibody,|Histone lysine demethylase PHF8 antibody|JHDM1F antibody|Jumonji C domain containing histone demethylase 1F antibody|MRXSSD antibody|PHD finger protein 8 antibody|PHF8 antibody|PHF8_HUMAN antibody|ZNF422 antibody|Anti-PHF8 antibody – ChIP Grade (ab36068)
SCBT cat No: sc-131751|
PHF8 Monoclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat, Dog, Pig
PHF8 Monoclonal Antibody detects endogenous levels of PHF8 protein.
Purified recombinant human PHF8 (C-terminus) protein fragments expressed in Ecoli
|Recommended dilution|| |
PHF8 Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
PHF8 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Histone lysine demethylase PHF8 antibody, PHD finger protein 8 antibody
|Protein names|| |
Histone lysine demethylase PHF8 , PHD finger protein 8
|Protein function|| |
Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 ‘Lys-9’ residue (H3K9Me1 and H3K9Me2), dimethylated H3 ‘Lys-27’ (H3K27Me2) and monomethylated histone H4 ‘Lys-20’ residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 ‘Lys-36’ (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated ‘Lys-4’ of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3. / Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2. / Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2. / Fe2+ / 134 µM for histone H3 H3K9Me2 / 8 µM for histone H3 H3K4me3 and H3K9Me2
|Involvement in disease|| |
Mental retardation, X-linked, syndromic, Siderius type (MRXSSD) [MIM:300263]: A syndrome characterized by mild to borderline mental retardation with or without cleft lip/cleft palate. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 promotes its access to H3K9me2. / The linker region is a critical determinant of demethylase specificity. It enables the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3. / Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. / Contains 1 JmjC domain. / Contains 1 PHD-type zinc finger.
|Protein post-translational modifications|| |
Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.
|Protein cellular localization|| |
Nucleus / Nucleus > nucleolus
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St John’s Laboratory Ltd.
|Product type|| |
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