Mouse Monoclonal phospho-MLKL (S358) antibody [6F8] (STJ97776)

$99.00$319.00

Reactivity: Human
Applications: IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal phospho-MLKL (S358) antibody [6F8] (STJ97776)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8).

Alternative names:

Check alternative names for the antibody

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MLKL antibody,|9130019I15Rik antibody|FLJ34389 antibody|hMLKL antibody|Mixed lineage kinase domain like antibody|Mixed lineage kinase domain like protein antibody|Mixed lineage kinase domain-like protein antibody|Mlkl antibody|MLKL_HUMAN antibody|Phospho anti-MLKL (S358) antibody [EPR9514] (ab187091)
SCBT cat No: sc-293201|sc-165024|sc-165025|

Name

phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8)

Catalogue No.

STJ97776

Reactivity

Human

Specificity

phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8) detects endogenous levels of MLKL

Immunogen

Synthetic Peptide of phospho-MLKL (S358)

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

phospho-MLKL (S358) Antibody(6F8) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

phospho-MLKL (S358) Mouse Antibody(6F8) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Mixed lineage kinase domain-like protein antibody, hMLKL antibody

Database links

Human UniProt/Swiss-Prot:Q8NB16;Mouse UniPort/Swiss-Prot: Q9D2Y4;Rat UniProt/Swiss-Port: D3ZKP6;Human Entrez Gene: 197259;Mouse Entrez Gene: 74568;

Protein names

Mixed lineage kinase domain-like protein , hMLKL

Protein function

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. / Inhibited by necrosulfonamide, a specific inhibitor of necroptosis that targets Cys-86.

Protein sequence and domain

The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity). / The coiled coil region 2 is responsible for homotrimerization. / Belongs to the protein kinase superfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

Protein cellular localization

Cytoplasm / Cell membrane

Note

AntibodyPlus can customize phospho-MLKL (S358) Antibody(6F8) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: phospho-MLKL (S358) Mouse Monoclonal Antibody(6F8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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