Mouse Monoclonal PRAK antibody [7H10B4] (STJ98335)

$379.00

Reactivity: Human
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal PRAK antibody [7H10B4] (STJ98335)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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MAPKAPK5 antibody, PRAK antibody,|MAP kinase-activated protein kinase 5 antibody|MAPK-activated protein kinase 5 antibody|MAPK5_HUMAN antibody|MAPKAP kinase 5 antibody|MAPKAPK-5 antibody|MAPKAPK5 antibody|Mitogen activated protein kinase activated protein kinase 5 antibody|p38-regulated/activated protein kinase antibody|PRAK antibody|Anti-PRAK antibody (ab97332)
SCBT cat No: sc-81705|sc-46667|sc-25419|sc-8251|

 

Name

PRAK Monoclonal Antibody

Catalogue No.

STJ98335

Reactivity

Human

Specificity

PRAK Monoclonal Antibody detects endogenous levels of PRAK protein.

Immunogen

Purified recombinant fragment of PRAK expressed in E Coli

Host

Mouse

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

PRAK Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

PRAK Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

MAP kinase-activated protein kinase 5 antibody, MAPK-activated protein kinase 5 antibody, MAPKAP kinase 5 antibody, MAPKAP-K5 antibody, MAPKAPK-5 antibody, MK-5 antibody, MK5 antibody, p38-regulated/activated protein kinase antibody, PRAK antibody

Database links

Human UniProt/Swiss-Prot:Q8IW41;Mouse UniPort/Swiss-Prot: O54992;Rat UniProt/Swiss-Port: F7FFP2;Human Entrez Gene: 8550;Mouse Entrez Gene: 17165;Rat Entrez Gene: Rn.17463

Protein names

MAP kinase-activated protein kinase 5 , MAPK-activated protein kinase 5 , MAPKAP kinase 5 , MAPKAP-K5 , MAPKAPK-5 , MK-5 , MK5 , p38-regulated/activated protein kinase , PRAK

Protein function

Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. / ATP + a protein = ADP + a phosphoprotein. / Activated following phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11, ERK2/MAPK1, ERK3/MAPK6, and ERK4/MAPK4. Activated by stress-related extracellular stimuli; such as H2O2, arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1. Inhibited by diterpenoid alkaloid noroxoaconitine.

Protein tissue specificity

Expressed ubiquitously.

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4; which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated (By similarity).

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>PRAK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize PRAK Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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