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Mouse Monoclonal PRAK antibody [7H10B4] (STJ98335)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
MAPKAPK5 antibody, PRAK antibody,|MAP kinase-activated protein kinase 5 antibody|MAPK-activated protein kinase 5 antibody|MAPK5_HUMAN antibody|MAPKAP kinase 5 antibody|MAPKAPK-5 antibody|MAPKAPK5 antibody|Mitogen activated protein kinase activated protein kinase 5 antibody|p38-regulated/activated protein kinase antibody|PRAK antibody|Anti-PRAK antibody (ab97332)
SCBT cat No: sc-81705|sc-46667|sc-25419|sc-8251|
PRAK Monoclonal Antibody
|Catalogue No.|| |
PRAK Monoclonal Antibody detects endogenous levels of PRAK protein.
Purified recombinant fragment of PRAK expressed in E Coli
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000
PRAK Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
PRAK Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
MAP kinase-activated protein kinase 5 antibody, MAPK-activated protein kinase 5 antibody, MAPKAP kinase 5 antibody, MAPKAP-K5 antibody, MAPKAPK-5 antibody, MK-5 antibody, MK5 antibody, p38-regulated/activated protein kinase antibody, PRAK antibody
|Protein names|| |
MAP kinase-activated protein kinase 5 , MAPK-activated protein kinase 5 , MAPKAP kinase 5 , MAPKAP-K5 , MAPKAPK-5 , MK-5 , MK5 , p38-regulated/activated protein kinase , PRAK
|Protein function|| |
Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. / ATP + a protein = ADP + a phosphoprotein. / Activated following phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11, ERK2/MAPK1, ERK3/MAPK6, and ERK4/MAPK4. Activated by stress-related extracellular stimuli; such as H2O2, arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1. Inhibited by diterpenoid alkaloid noroxoaconitine.
|Protein tissue specificity|| |
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4; which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated (By similarity).
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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