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Mouse Monoclonal PRMT4 antibody [3H2] (STJ98337)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, FC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
CARM1 antibody, PRMT4 antibody,|carm1 antibody|CARM1_HUMAN antibody|Coactivator associated arginine methyltransferase 1 antibody|Coactivator-associated arginine methyltransferase 1 antibody|Histone arginine methyltransferase CARM 1 antibody|Histone arginine methyltransferase CARM1 antibody|Histone-arginine methyltransferase CARM1 antibody|PRMT 4 antibody|PRMT4 antibody|Protein arginine methyltransferase antibody|Protein arginine N methyltransferase 4 antibody|Protein arginine N-methyltransferase 4 antibody|Anti-PRMT4 antibody (ab128851)
SCBT cat No: sc-390656|sc-393381|sc-33176|sc-5421|sc-5418|
PRMT4 Monoclonal Antibody
|Catalogue No.|| |
Human, Rat, Monkey
PRMT4 Monoclonal Antibody detects endogenous levels of PRMT4 protein.
Purified recombinant fragment of human PRMT4 expressed in E Coli
WB, IHC, IF, FC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
PRMT4 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
PRMT4 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Histone-arginine methyltransferase CARM1 antibody, Coactivator-associated arginine methyltransferase 1 antibody, Protein arginine N-methyltransferase 4 antibody
|Protein names|| |
Histone-arginine methyltransferase CARM1 , Coactivator-associated arginine methyltransferase 1 , Protein arginine N-methyltransferase 4
|Protein function|| |
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at ‘Arg-17’ (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at ‘Arg-2142’, which may loosen its interaction with NCOA2/GRIP1, and at ‘Arg-580’ and ‘Arg-604’ in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. / 2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega)-dimethyl-L-arginine. / Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 ‘Lys-18’ (H3K18ac) H3 ‘Lys-23’ (H3K23ac) by EP300 and blocked by citrullination of H3 ‘Arg-17’ (H3R17ci) by PADI4.
|Protein tissue specificity|| |
Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
|Protein sequence and domain|| |
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. / Contains 1 SAM-dependent MTase PRMT-type domain.
|Protein post-translational modifications|| |
Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity). / Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
|Protein cellular localization|| |
Nucleus / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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