Mouse Monoclonal PRMT4 antibody (STJ98541)

$379.00

Reactivity: Human, Mouse, Rat, Cow, Pig
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Mouse Monoclonal PRMT4 antibody (STJ98541)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for PRMT4 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

CARM1 antibody, PRMT4 antibody,|carm1 antibody|CARM1_HUMAN antibody|Coactivator associated arginine methyltransferase 1 antibody|Coactivator-associated arginine methyltransferase 1 antibody|Histone arginine methyltransferase CARM 1 antibody|Histone arginine methyltransferase CARM1 antibody|Histone-arginine methyltransferase CARM1 antibody|PRMT 4 antibody|PRMT4 antibody|Protein arginine methyltransferase antibody|Protein arginine N methyltransferase 4 antibody|Protein arginine N-methyltransferase 4 antibody|Anti-PRMT4 antibody (ab128851)
SCBT cat No: sc-390656|sc-393381|sc-33176|sc-5421|sc-5418|

 

Name

PRMT4 Monoclonal Antibody

Catalogue No.

STJ98541

Reactivity

Human, Mouse, Rat, Cow, Pig

Specificity

PRMT4 Monoclonal Antibody detects endogenous levels of PRMT4 protein.

Immunogen

Purified recombinant human PRMT4 (C-terminus) protein fragments expressed in Ecoli

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

PRMT4 Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Concentration

1 mg/ml

Purification

PRMT4 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Histone-arginine methyltransferase CARM1 antibody, Coactivator-associated arginine methyltransferase 1 antibody, Protein arginine N-methyltransferase 4 antibody

Database links

Human UniProt/Swiss-Prot:Q86X55;Mouse UniPort/Swiss-Prot: Q9WVG6;Rat UniProt/Swiss-Port: Q4AE70;Human Entrez Gene: 10498;Mouse Entrez Gene: 59035;Rat Entrez Gene: Rn.94869

Protein names

Histone-arginine methyltransferase CARM1 , Coactivator-associated arginine methyltransferase 1 , Protein arginine N-methyltransferase 4

Protein function

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at ‘Arg-17’ (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at ‘Arg-2142’, which may loosen its interaction with NCOA2/GRIP1, and at ‘Arg-580’ and ‘Arg-604’ in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. / 2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega)-dimethyl-L-arginine. / Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 ‘Lys-18’ (H3K18ac) H3 ‘Lys-23’ (H3K23ac) by EP300 and blocked by citrullination of H3 ‘Arg-17’ (H3R17ci) by PADI4.

Protein tissue specificity

Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Protein sequence and domain

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. / Contains 1 SAM-dependent MTase PRMT-type domain.

Protein post-translational modifications

Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity). / Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.

Protein cellular localization

Nucleus / Cytoplasm

Research area

All research areas>Transcription Regulators>CARM
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize PRMT4 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!