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Mouse Monoclonal PYK2 antibody (STJ98544)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB
Recommended dilution: WB 1:1000-1:2000
Recommended protocols: check protocols
Click or hover above images to see image description for PYK2 Monoclonal Antibody.
Check alternative names for the antibodyExpand
PTK2B antibody, FAK2 antibody, PYK2 antibody, RAFTK antibody,|CADTK antibody||Anti-PYK2 antibody [YE353] (ab32571)
SCBT cat No: sc-293142|sc-81512|sc-101790|sc-11767|sc-16822|sc-16824|sc-16823|sc-16825|sc-374260|sc-26054|sc-32910|sc-376039|sc-26052|sc-241639|sc-130071|sc-398898|
PYK2 Monoclonal Antibody
|Catalogue No.|| |
Human, Mouse, Dog, Pig, Rabbit
PYK2 Monoclonal Antibody detects endogenous levels of PYK2 protein.
Purified recombinant human PYK2 protein fragments expressed in Ecoli
|Recommended dilution|| |
PYK2 Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
PYK2 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Protein-tyrosine kinase 2-beta antibody, Calcium-dependent tyrosine kinase antibody, CADTK antibody, Calcium-regulated non-receptor proline-rich tyrosine kinase antibody, Cell adhesion kinase beta antibody, CAK-beta antibody, CAKB antibody, Focal adhesion kinase 2 antibody, FADK 2 antibody, Proline-rich tyrosine kinase 2 antibody, Related adhesion focal tyrosine kinase antibody, RAFTK antibody
|Protein names|| |
Protein-tyrosine kinase 2-beta , Calcium-dependent tyrosine kinase , CADTK , Calcium-regulated non-receptor proline-rich tyrosine kinase , Cell adhesion kinase beta , CAK-beta , CAKB , Focal adhesion kinase 2 , FADK 2 , Proline-rich tyrosine kinase 2 , Related adhesion focal tyrosine kinase , RAFTK
|Protein function|| |
Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at ‘Tyr-9, ‘Tyr-373’, and ‘Tyr-376’. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Activated in response to stimuli that lead to increased intracellular Ca2+ levels; this activation is indirect and may be mediated by calcium-mediated production of reactive oxygen species (ROS). Activated by autophosphorylation at Tyr-402; this creates a binding site for SRC family kinases and leads to phosphorylation at additional tyrosine residues. Phosphorylation at Tyr-402, Tyr-579 and Tyr-580 is required for optimal kinase activity. Inhibited by PF-562,271, BIRB796, PF-4618433 and by PF-431396, PF-2318841 and their derivatives. Inhibited by sulfoximine-substituted trifluoromethylpyrimidines. Inhibited by 4-amino and 5-aryl substituted pyridinone compounds.
|Protein tissue specificity|| |
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
|Involvement in disease|| |
Note: Aberrant PTK2B/PYK2 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. Elevated PTK2B/PYK2 expression is seen in gliomas, hepatocellular carcinoma, lung cancer and breast cancer.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. / Contains 1 FERM domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration; this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579; Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.
|Protein cellular localization|| |
Cytoplasm / Cytoplasm > perinuclear region / Cell membrane; Peripheral membrane protein; Cytoplasmic side / Cell junction > focal adhesion / Cell projection > lamellipodium / Cytoplasm > cell cortex / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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