Mouse Monoclonal RUNX1 antibody [2B5] (STJ98372)

$379.00

Reactivity: Human
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal RUNX1 antibody [2B5] (STJ98372)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for RUNX1 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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RUNX1 antibody, AML1 antibody, CBFA2 antibody,|Acute myeloid leukemia 1 antibody|RUNX 1 antibody|Runx1 antibody|RUNX1_HUMAN antibody|SL3 3 enhancer factor 1 alpha B subunit antibody|SL3-3 enhancer factor 1 alpha B subunit antibody|SL3/AKV core binding factor alpha B subunit antibody|SL3/AKV core-binding factor alpha B subunit antibody|Anti-RUNX1 / AML1 antibody – ChIP Grade (ab23980)
SCBT cat No: sc-293146|sc-137150|sc-137222|sc-271047|sc-271020|sc-137212|

 

Name

RUNX1 Monoclonal Antibody

Catalogue No.

STJ98372

Reactivity

Human

Specificity

RUNX1 Monoclonal Antibody detects endogenous levels of RUNX1 protein.

Immunogen

Synthesized peptide of human RUNX1

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

RUNX1 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

RUNX1 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Runt-related transcription factor 1 antibody, Acute myeloid leukemia 1 protein antibody, Core-binding factor subunit alpha-2 antibody, CBF-alpha-2 antibody, Oncogene AML-1 antibody, Polyomavirus enhancer-binding protein 2 alpha B subunit antibody, PEA2-alpha B antibody, PEBP2-alpha B antibody, SL3-3 enhancer factor 1 alpha B subunit antibody, SL3/AKV core-binding factor alpha B subunit antibody

Database links

Human UniProt/Swiss-Prot:Q01196;Rat UniProt/Swiss-Port: Q63046;Human Entrez Gene: 861;Mouse Entrez Gene: 12394;Rat Entrez Gene: Rn.11201

Protein names

Runt-related transcription factor 1 , Acute myeloid leukemia 1 protein , Core-binding factor subunit alpha-2 , CBF-alpha-2 , Oncogene AML-1 , Polyomavirus enhancer-binding protein 2 alpha B subunit , PEA2-alpha B , PEBP2-alpha B , SL3-3 enhancer factor 1 alpha B subunit , SL3/AKV core-binding factor alpha B subunit

Protein function

CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells .

Protein tissue specificity

Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Involvement in disease

Note: A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.; Note: A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.; Note: A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.; Note: A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3′-end of TEL to the alternate 5′-exon of AML-1H.; Note: A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.; Familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]: Autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Note: A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.; Note: A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.; Note: A chromosomal aberration involving RUNX1/AML1 is found in acute myeloid leukemia. Translocation t(20;21)(q11;q22) with CBFA2T2. .

Protein sequence and domain

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes. / Contains 1 Runt domain.

Protein post-translational modifications

Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A. / Methylated. / Phosphorylated in Ser-249 Thr-273 and Ser-276 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>RUNX
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize RUNX1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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