Mouse Monoclonal TIF1beta antibody (STJ98563)

$379.00

Reactivity: Human, Mouse, Rat, Dog, Rabbit
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal TIF1beta antibody (STJ98563)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-1:2000

Recommended protocols: check protocols

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Alternative names:

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TRIM28 antibody, KAP1 antibody, RNF96 antibody, TIF1B antibody,|E3 SUMO protein ligase TRIM28 antibody|E3 SUMO-protein ligase TRIM28 antibody|FLJ29029 antibody|KAP 1 antibody|KAP-1 antibody|KRAB associated protein 1 antibody|KRAB interacting protein 1 antibody|KRAB-associated protein 1 antibody|KRAB-interacting protein 1 antibody|KRIP 1 antibody|KRIP-1 antibody|KRIP1 antibody|Nuclear corepressor KAP 1 antibody|Nuclear corepressor KAP-1 antibody|RING finger protein 96 antibody|RNF96 antibody|TF1B antibody|TIF1 beta antibody|TIF1-beta antibody|TIF1B antibody|TIF1B_HUMAN antibody|Transcription intermediary factor 1 beta antibody|Transcription intermediary factor 1-beta antibody|Trim28 antibody|Tripartite motif containing 28 antibody|tripartite motif containing protein 28 antibody|Tripartite motif-containing protein 28 antibody|Anti-KAP1 antibody – ChIP Grade (ab10483)
SCBT cat No: To be updated

 

Name

TIF1beta Monoclonal Antibody

Catalogue No.

STJ98563

Reactivity

Human, Mouse, Rat, Dog, Rabbit

Specificity

TIF1beta Monoclonal Antibody detects endogenous levels of TIF1beta protein.

Immunogen

Purified recombinant human TIF1beta (C-terminus) protein fragments expressed in Ecoli

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

TIF1beta Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Concentration

1 mg/ml

Purification

TIF1beta Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Transcription intermediary factor 1-beta antibody, TIF1-beta antibody, E3 SUMO-protein ligase TRIM28 antibody, KRAB-associated protein 1 antibody, KAP-1 antibody, KRAB-interacting protein 1 antibody, KRIP-1 antibody, Nuclear corepressor KAP-1 antibody, RING finger protein 96 antibody, Tripartite motif-containing protein 28 antibody

Database links

Human UniProt/Swiss-Prot:Q13263;Mouse UniPort/Swiss-Prot: Q62318;Rat UniProt/Swiss-Port: O08629;Human Entrez Gene: 10155;Mouse Entrez Gene: 21849;Rat Entrez Gene: Rn.198494

Protein names

Transcription intermediary factor 1-beta , TIF1-beta , E3 SUMO-protein ligase TRIM28 , KRAB-associated protein 1 , KAP-1 , KRAB-interacting protein 1 , KRIP-1 , Nuclear corepressor KAP-1 , RING finger protein 96 , Tripartite motif-containing protein 28

Protein function

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at ‘Lys-9’ (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 ‘Lys-9 and ‘Lys-14’ acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) . Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells . Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) . Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing . /

Protein tissue specificity

Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Protein sequence and domain

The HP1 box is both necessary and sufficient for HP1 binding. / The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain. / The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization. / Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. / Belongs to the TRIM/RBCC family. / Contains 2 B box-type zinc fingers. / Contains 1 bromo domain. / Contains 1 PHD-type zinc finger. / Contains 1 RING-type zinc finger.

Protein post-translational modifications

ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes. / Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21. / Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2. / Citrullinated by PADI4.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>TIF1
(View all antibody categories related to Transcription Regulators)

Note

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Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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