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Mouse Monoclonal TRIM5alpha antibody [3B11H2] (STJ98429)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000
Recommended protocols: check protocols
Click or hover above images to see image description for TRIM5alpha Monoclonal Antibody.
Check alternative names for the antibodyExpand
TRIM5 antibody, RNF88 antibody,|RING finger protein 88 antibody|RNF88 antibody|TRIM5 antibody|TRIM5_HUMAN antibody|tripartite motif protein TRIM5 antibody|Tripartite motif-containing protein 5 antibody|Anti-TRIM5 alpha antibody (ab4389)
SCBT cat No: To be updated
TRIM5alpha Monoclonal Antibody
|Catalogue No.|| |
TRIM5alpha Monoclonal Antibody detects endogenous levels of TRIM5alpha protein.
Purified recombinant fragment of human TRIM5alpha expressed in E Coli
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000
TRIM5alpha Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
TRIM5alpha Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Tripartite motif-containing protein 5 antibody, RING finger protein 88 antibody
|Protein names|| |
Tripartite motif-containing protein 5 , RING finger protein 88
|Protein function|| |
Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates ‘Lys-63’-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV) . Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 . Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction . /
|Protein sequence and domain|| |
The B box-type zinc finger domain and the coiled-coil domain contribute to the higher and low order multimerization respectively which is essential for restriction activity (PubMed:22482711). The coiled coil domain is important for higher order multimerization by promoting the initial dimerization (By similarity). / The B30.2/SPRY domain acts as a capsid recognition domain. Polymorphisms in this domain explain the observed species-specific differences among orthologs (PubMed:22482711). / The RING-type zinc finger domain confers E3 ubiquitin ligase activity and is essential for retrovirus restriction activity, autoubiquitination and higher-order multimerization. / Belongs to the TRIM/RBCC family. / Contains 1 B box-type zinc finger. / Contains 1 B30.2/SPRY domain. / Contains 1 RING-type zinc finger.
|Protein post-translational modifications|| |
Degraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus. / Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation.
|Protein cellular localization|| |
Cytoplasm / Cytoplasm > P-body / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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