Mouse Monoclonal Vimentin antibody [4F2E9] (STJ98448)


Reactivity: Human, Monkey
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Vimentin antibody [4F2E9] (STJ98448)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Vimentin Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody


VIM antibody,|CTRCT30 antibody|Epididymis luminal protein 113 antibody|FLJ36605 antibody|HEL113 antibody|VIM antibody|VIME_HUMAN antibody|Vimentin antibody|Anti-Vimentin antibody [V9] – Cytoskeleton Marker (ab8069)
SCBT cat No: sc-16673|sc-12973|sc-16674|sc-248328|sc-248326|sc-166656|sc-390188|sc-398474|sc-12934|sc-377077|sc-48403|sc-48340|sc-25381|sc-9690|sc-7739|sc-7737|sc-66913|



Vimentin Monoclonal Antibody

Catalogue No.



Human, Monkey


Vimentin Monoclonal Antibody detects endogenous levels of Vimentin protein.


Purified recombinant fragment of Vimentin (aa2-466) expressed in E Coli





Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000








Vimentin Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.


1 mg/ml


Vimentin Antibody was purified using affinity purification.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Vimentin antibody

Database links

Human UniProt/Swiss-Prot:P08670;Mouse UniPort/Swiss-Prot: P20152;Rat UniProt/Swiss-Port: P31000;Human Entrez Gene: 7431;Mouse Entrez Gene: 22352;Rat Entrez Gene: Rn.2710

Protein names


Protein function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. / Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Protein tissue specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt’s lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Involvement in disease

Cataract 30 (CTRCT30) [MIM:116300]: An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The central alpha-helical coiled-coil rod region mediates elementary homodimerization. / The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. / Belongs to the intermediate filament family.

Protein post-translational modifications

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. / O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. / S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Protein cellular localization


Research area

All research areas>Structural Proteins>Vimentin
(View all antibody categories related to Structural Proteins)


AntibodyPlus can customize Vimentin Antibody according to your requirement, including bulk product size,etc. Please contact AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


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