Mouse Monoclonal XRCC4 antibody [5C10] (STJ96988)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal XRCC4 antibody [5C10] (STJ96988)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IP

Recommended dilution: WB 1:2000; IP 1:200

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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XRCC4 antibody,|DNA double strand break repair and V(D)J recombination protein XRCC4 antibody|DNA repair protein XRCC4 antibody|SSMED antibody|X ray repair complementing defective repair in Chinese hamster cells 4 antibody|X ray repair cross complementing 4 antibody|X ray repair cross complementing protein 4 antibody|X-ray repair cross-complementing protein 4 antibody|XRCC 4 antibody|XRCC4 antibody|XRCC4_HUMAN antibody|Anti-XRCC4 antibody [4H9] (ab118008)
SCBT cat No: To be updated

Name

XRCC4 Monoclonal Antibody

Catalogue No.

STJ96988

Reactivity

Human

Specificity

The antibody detects endogenous XRCC4 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IP

Recommended dilution

WB 1:2000; IP 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

XRCC4 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

XRCC4 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

DNA repair protein XRCC4 antibody, X-ray repair cross-complementing protein 4 antibody

Database links

Human UniProt/Swiss-Prot:Q13426;Mouse UniPort/Swiss-Prot: Q924T3;Rat UniProt/Swiss-Port: Q5XI44;Human Entrez Gene: 7518;Mouse Entrez Gene: 108138;Rat Entrez Gene: Rn.40979

Protein names

DNA repair protein XRCC4 , X-ray repair cross-complementing protein 4

Protein function

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Protein tissue specificity

Widely expressed.

Involvement in disease

Short stature, microcephaly, and endocrine dysfunction (SSMED) [MIM:616541]: A disease characterized by short stature and microcephaly apparent at birth, progressive post-natal growth failure, and endocrine dysfunction. In affected adults endocrine features include hypergonadotropic hypogonadism, multinodular goiter, and diabetes mellitus. Variable features observed in some patients are progressive ataxia, and lymphopenia or borderline leukopenia. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the XRCC4 family.

Protein post-translational modifications

Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX. / Monoubiquitinated. / Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.

Protein cellular localization

Nucleus

Research area

All research areas>Tumor Suppressor/Apoptosis>XRCC
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize XRCC4 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

1 review for Mouse Monoclonal XRCC4 antibody [5C10] (STJ96988)


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Immunofluorescence analysis of Human liver cancer tissue

1: XRCC4 Monoclonal Antibody(5C10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: XRCC4 Monoclonal Antibody(5C10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: XRCC4 Monoclonal Antibody(5C10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: XRCC4 Monoclonal Antibody(5C10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: XRCC4 Monoclonal Antibody(5C10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: XRCC4 Monoclonal Antibody(5C10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue

1: XRCC4 Monoclonal Antibody(5C10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: XRCC4 Monoclonal Antibody(5C10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: XRCC4 Monoclonal Antibody(5C10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: XRCC4 Monoclonal Antibody(5C10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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