Mouse TNF alpha ELISA Kit (ARG80206)

$499.00

Size: 96 wells
Conjugation: HRP
Supplier: Arigo Biolaboratories

Overview


Supplier : Arigo Biolaboratories

 Production type ELISA Kits
Tested Reactivity Mouse
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human Lymphotoxin alpha1/ beta2, Lymphotoxin alpha2/ beta1, TNF- alpha, TNF RI, TNF RII
Target Name TNF alpha
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 8 pg/ml
Detection Range 15.6-1000 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Sample Volume 100 ul
Precision

CV: <10%

Full Name tumor necrosis factor
Alternate Names Check alternative names for the Mouse TNF alpha ELISA Kit

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Application Instructions

 

Assay Time 4 hours


Properties

 

Form 96 wells
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components or simply ask Antibodyplus for ELISA troublesooting.
Note Antibodyplus and Arigo provide the most validated ELISA kits for researchers. For laboratory research only, not for drug, diagnostic or other use.
User’s manual of Mouse TNF alpha ELISA Kit  Download
size

96 wells

Cat No.

ARG80206

Conjugation note

Substrate: TMB and read at 450 nm

Sensitivity

8 pg/ml

Detection range

15.6-1000 pg/ml

Sample type

Serum, Plasma, Cell culture supernatants

Sample Volume

100 ul

Precision

CV: <10%

Target name

TNF alpha

Assay time

4 hours

Store at

4 Celcius degree

Storage instruction

Store the kit at 2-8 Celcius degree. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Background

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

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