Rabbit polyclonal ABL1 antibody (A0282)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ABL1 antibody (A0282)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ABL1 antibody,ABL antibody,JTK7 antibody,bcr/abl antibody,c-ABL antibody,p150 antibody,v-abl antibody
Abelson murine leukemia viral oncogene homolog 1 antibody|Abelson tyrosine protein kinase 1 antibody|Abl 1 antibody|ABL antibody|ABL proto oncogene 1 non receptor tyrosine kinase antibody|ABL1 antibody|ABL1_HUMAN antibody|bcr/abl antibody|bcr/c abl oncogene protein antibody|c ABL antibody|c abl oncogene 1 non receptor tyrosine kinase antibody|c abl oncogene 1 receptor tyrosine kinase antibody|c ABL1 antibody|JTK7 antibody|p150 antibody|Proto oncogene tyrosine protein kinase ABL1 antibody|Proto-oncogene c-Abl antibody|Tyrosine-protein kinase ABL1 antibody|v abl Abelson murine leukemia viral oncogene homolog 1 antibody|v abl antibody|Phospho anti-c Abl (Y412) antibody (ab47315)

SCBT cat No: sc-887|sc-13076|sc-131|sc-52990|sc-374316|sc-33597|sc-376272|

Name

Rabbit polyclonal ABL1 antibody

Catalogue No.

A0282

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human ABL1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:100 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 123kDa/Observed: Refer to Figures

Formulation

ABL1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ABL1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ABL1 antibody,ABL antibody,JTK7 antibody,bcr/abl antibody,c-ABL antibody,p150 antibody,v-abl antibody

Database links

Human UniProt/Swiss-Prot:P00519

Protein names

ABL1,ABL,JTK7,bcr/abl,c-ABL,p150,v-abl

Protein function

Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on ‘Tyr-153’ and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Protein tissue specificity

Widely expressed.

Protein sequence and domain

Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.; Contains 1 protein kinase domain.; Contains 1 SH2 domain.; Contains 1 SH3 domain.

Protein post-translational modifications

Acetylated at Lys-711 by EP300 which promotes the cytoplasmic translocation.; Phosphorylation at Tyr-70 by members of the SRC family of kinases disrupts SH3 domain-based autoinhibitory interactions and intermolecular associations, such as that with ABI1, and also enhances kinase activity. Phosphorylation at Tyr-226 and Tyr-393 correlate with increased activity. DNA damage-induced activation of ABL1 requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation at Ser-569 has been attributed to a CDC2-associated kinase and is coupled to cell division (By similarity). Phosphorylation at Ser-618 and Ser-619 by PAK2 increases binding to CRK and reduces binding to ABI1. Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation. Phosphorylated by PRKDC (By similarity).; Polyubiquitinated. Polyubiquitination of ABL1 leads to degradation.

Protein cellular localization

Cytoplasm, cytoskeleton. Nucleus. Mitochondrion . Note: Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity).; Isoform IB: Nucleus membrane; Lipid-anchor. Note: The myristoylated c-ABL protein is reported to be nuclear.

Background

The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).

Research area

All research areas>Kinases and Phosphatases>c-Abl
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize ABL1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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