Rabbit Polyclonal Acetyl-Histone H2B (K12) antibody (STJ90119)

$99.00$319.00

Reactivity: Human, Mouse, Monkey
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Acetyl-Histone H2B (K12) antibody (STJ90119)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Acetyl Histone H2B (K12) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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H2BFS antibody,||
SCBT cat No: To be updated

 

Name

Acetyl Histone H2B (K12) Polyclonal Antibody

Catalogue No.

STJ90119

Reactivity

Human, Mouse, Monkey

Specificity

Acetyl-Histone H2B (K12) Polyclonal Antibody detects endogenous levels of Histone H2B protein only when acetylated at K12.

Immunogen

Synthesized acetyl-peptide derived from Acetyl Histone H2B (K12) at AA range 10-90

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

15 kDa

Formulation

Acetyl Histone H2B (K12) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Acetyl Histone H2B (K12) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Histone H2B type F-S antibody, Histone H2B.s antibody, H2B/s antibody

Database links

Human UniProt/Swiss-Prot:P57053;Rat UniProt/Swiss-Port: G3V8B3;Human Entrez Gene: 54145;

Protein names

Histone H2B type F-S , Histone H2B.s , H2B/s

Protein function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. / Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Protein sequence and domain

Belongs to the histone H2B family.

Protein post-translational modifications

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 ‘Lys-4’ (H3K4me) and ‘Lys-79’ (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 ‘Lys-4’ and ‘Lys-79’ methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. / Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. / GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity). / Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Protein cellular localization

Nucleus / Chromosome

Research area

All research areas>Transcription Regulators>Histone
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Acetyl Histone H2B (K12) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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