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Rabbit Polyclonal Acetyl-Ref-1 (K6) antibody (STJ90137)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;
Recommended protocols: check protocols
Click or hover above images to see image description for Ref-1 (Acetyl Lys6) Polyclonal Antibody.
Check alternative names for the antibodyExpand
APEX1 antibody, APE antibody, APE1 antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody,|AP endonuclease 1 antibody|AP endonuclease class I antibody|AP lyase antibody|APE 1 antibody|APE antibody|APE-1 antibody|APEN antibody|APEX 1 antibody|APEX antibody|APEX nuclease (multifunctional DNA repair enzyme) 1 antibody|Apex nuclease 1 antibody|APEX nuclease antibody|APEX1 antibody|APEX1_HUMAN antibody|Apurinic endonuclease antibody|Apurinic-apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic (abasic) endonuclease antibody|Apurinic/apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic exonuclease antibody|APX antibody|BAP1 antibody|Deoxyribonuclease (apurinic or apyrimidinic) antibody|DNA (apurinic or apyrimidinic site) lyase antibody|DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody|EC 188.8.131.52 antibody|HAP 1 antibody|HAP1 antibody|Human Apurinic endonuclease 1 antibody|MGC139790 antibody|Multifunctional DNA repair enzyme antibody|Redox factor 1 antibody|Redox factor-1 antibody|REF 1 antibody|REF 1 protein antibody|REF-1 antibody|REF1 antibody|REF1 protein antibody|Anti-APE1 antibody [13B8E5C2] – ChIP Grade (ab194)
SCBT cat No: sc-136057|sc-334|sc-17774|sc-9919|sc-5572|sc-55498|
Ref-1 (Acetyl Lys6) Polyclonal Antibody
|Catalogue No.|| |
Acetyl-Ref-1 (K6) Polyclonal Antibody detects endogenous levels of Ref-1 protein only when acetylated at K6.
Synthesized acetyl-peptide derived from the human Ref-1 around the acetylation site of K6
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC-P 1:100-300; ELISA 1:20000;
|Molecular weight|| |
Ref-1 (Acetyl Lys6) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Ref-1 (Acetyl Lys6) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
DNA-antibody, apurinic or apyrimidinic site antibody, lyase antibody, APEX nuclease antibody, APEN antibody, Apurinic-apyrimidinic endonuclease 1 antibody, AP endonuclease 1 antibody, APE-1 antibody, REF-1 antibody, Redox factor-1 antibody
|Protein names|| |
DNA-, apurinic or apyrimidinic site , lyase , APEX nuclease , APEN , Apurinic-apyrimidinic endonuclease 1 , AP endonuclease 1 , APE-1 , REF-1 , Redox factor-1
|Protein function|| |
Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5′-deoxyribose phosphate and 3′-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3′-5′ exoribonuclease activity on mismatched deoxyribonucleotides at the 3′ termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3′ phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3′ side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5′-terminal deoxyribose 5′-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA. / The C-O-P bond 3′ to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3′-terminal unsaturated sugar and a product with a terminal 5′-phosphate. / Mg2+ / NPM1 stimulates endodeoxyribonuclease activity on double-stranded DNA with AP sites, but inhibits endoribonuclease activity on single-stranded RNA containing AP sites.
|Protein tissue specificity|| |
Up-regulated in presence of reactive oxygen species (ROS), like bleomycin, H2O2 and phenazine methosulfate.
|Protein sequence and domain|| |
The N-terminus contains the redox activity while the C-terminus exerts the DNA AP-endodeoxyribonuclease activity; both function are independent in their actions. An unconventional mitochondrial targeting sequence (MTS) is harbored within the C-terminus, that appears to be masked by the N-terminal sequence containing the nuclear localization signal (NLS), that probably blocks the interaction between the MTS and Tom proteins. / Belongs to the DNA repair enzymes AP/ExoA family.
|Protein post-translational modifications|| |
Phosphorylated. Phosphorylation by kinase PKC or casein kinase CK2 results in enhanced redox activity that stimulates binding of the FOS/JUN AP-1 complex to its cognate binding site. AP-endodeoxyribonuclease activity is not affected by CK2-mediated phosphorylation. Phosphorylation of Thr-233 by CDK5 reduces AP-endodeoxyribonuclease activity resulting in accumulation of DNA damage and contributing to neuronal death. / Acetylated on Lys-6 and Lys-7. Acetylation is increased by the transcriptional coactivator EP300 acetyltransferase, genotoxic agents like H2O2 and methyl methanesulfonate (MMS). Acetylation increases its binding affinity to the negative calcium response element (nCaRE) DNA promoter. The acetylated form induces a stronger binding of YBX1 to the Y-box sequence in the MDR1 promoter than the unacetylated form. Deacetylated on lysines. Lys-6 and Lys-7 are deacetylated by SIRT1. / Cleaved at Lys-31 by granzyme A to create the mitochondrial form; leading in reduction of binding to DNA, AP endodeoxynuclease activity, redox activation of transcription factors and to enhanced cell death. Cleaved by granzyme K; leading to intracellular ROS accumulation and enhanced cell death after oxidative stress. / Cys-65 and Cys-93 are nitrosylated in response to nitric oxide (NO) and lead to the exposure of the nuclear export signal (NES). / Ubiquitinated by MDM2; leading to translocation to the cytoplasm and proteasomal degradation.
|Protein cellular localization|| |
Nucleus / Nucleus > nucleolus / Nucleus speckle / Endoplasmic reticulum / Cytoplasm / Mitochondrion
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St John’s Laboratory Ltd.
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