Rabbit Polyclonal AGO2 antibody (A6802)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal AGO2 antibody (A6802)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IP

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:100 – 1:100
IP 1:20 – 1:50

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

Q10 antibody; AGO2
Ago 2 antibody|AGO2_HUMAN antibody|Argonaute 2 antibody|argonaute 2, RISC catalytic component antibody|Argonaute RISC catalytic component 2 antibody|Argonaute2 antibody|CTA-204B4.6 antibody|dAgo2 antibody|eIF 2C 2 antibody|eIF-2C 2 antibody|eIF2C 2 antibody|Eif2c2 antibody|Eukaryotic translation initiation factor 2C 2 antibody|Eukaryotic translation initiation factor 2C subunit 2 antibody|hAgo2 antibody|MGC3183 antibody|PAZ Piwi domain protein antibody|PPD antibody|Protein argonaute-2 antibody|Protein slicer antibody|Q10 antibody|Slicer protein antibody|Anti-Argonaute-2 antibody – ChIP Grade (ab57113)

SCBT cat No: sc-53521|sc-32659|

Name

Rabbit Polyclonal AGO2 antibody

Catalogue No.

A6802

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human AGO2

Host

Rabbit

Applications

WB, IHC, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:100 – 1:100
IP 1:20 – 1:50

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

97kDa

Formulation

AGO2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

Q10 antibody; AGO2

Database links

Human UniProt/Swiss-Prot:Q9UKV8

Protein names

Q10; AGO2

Protein function

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The ‘minimal RISC’ appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3′ untranslated region (3′-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3′-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Protein sequence and domain

Belongs to the argonaute family. Ago subfamily.; Contains 1 PAZ domain.; Contains 1 Piwi domain. The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However, while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).

Protein post-translational modifications

Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.

Protein cellular localization

Cytoplasm, P-body. Nucleus. Note=Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.

Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.

Note

AntibodyPlus can customize AGO2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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