Rabbit Polyclonal Akt1 antibody (STJ91542)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Akt1 antibody (STJ91542)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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AKT1 antibody, PKB antibody, RAC antibody,|AKT 1 antibody|AKT 2 antibody|AKT antibody|AKT1 antibody|AKT1 kinase antibody|AKT1m antibody|AKT2 antibody|AKT2 kinase antibody|C AKT antibody|CWS6 antibody|HIHGHH antibody|MGC99656 antibody|Murine thymoma viral (v akt) homolog 2 antibody|Oncogene AKT1 antibody|PKB alpha antibody|PKB antibody|PKB beta antibody|PKBB antibody|PKBBETA antibody|PRKBA antibody|PRKBB antibody|Protein kinase Akt 2 antibody|Protein Kinase B Alpha antibody|Protein kinase B antibody|Protein kinase B beta antibody|Proto oncogene c Akt antibody|RAC antibody|RAC alpha antibody|RAC alpha serine/threonine protein kinase antibody|RAC BETA antibody|RAC beta serine threonine protein kinase antibody|RAC PK alpha antibody|RAC PK beta antibody|Rac protein kinase alpha antibody|Rac protein kinase beta antibody|RACbeta antibody|v akt murine thymoma viral oncogene homolog 1 antibody|v akt murine thymoma viral oncogene homolog 2 antibody|Phospho anti-AKT1 (S473) antibody [EP2109Y] (ab81283)
SCBT cat No: sc-135829|sc-377457|sc-5298|sc-1618|sc-7126|sc-271149|sc-55523|sc-1619|sc-81434|sc-56878|sc-8312|sc-81148|sc-81436|sc-7127|sc-5270|sc-11520|sc-134254|sc-11521|sc-100321|sc-374073|sc-376133|sc-165991|sc-98547|sc-165992|sc-81712|

Name

Akt1 Polyclonal Antibody

Catalogue No.

STJ91542

Reactivity

Human, Mouse, Rat

Specificity

Akt1 Polyclonal Antibody detects endogenous levels of Akt1 protein.

Immunogen

Synthesized peptide derived from Akt1 at AA range 70-150

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

60 kDa

Formulation

Akt1 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Akt1 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

RAC-alpha serine/threonine-protein kinase antibody, Protein kinase B antibody, PKB antibody, Protein kinase B alpha antibody, PKB alpha antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody

Database links

Human UniProt/Swiss-Prot:P31749;Mouse UniPort/Swiss-Prot: P31750;Rat UniProt/Swiss-Port: P47196;Human Entrez Gene: 207;Mouse Entrez Gene: 11651;Rat Entrez Gene: Rn.11422

Protein names

RAC-alpha serine/threonine-protein kinase , Protein kinase B , PKB , Protein kinase B alpha , PKB alpha , Proto-oncogene c-Akt , RAC-PK-alpha

Involvement in disease

Breast cancer (BC) [MIM:114480]: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.; Colorectal cancer (CRC) [MIM:114500]: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. Note: The gene represented in this entry may be involved in disease pathogenesis.; Note: Genetic variations in AKT1 may play a role in susceptibility to ovarian cancer.; Proteus syndrome (PROTEUSS) [MIM:176920]: A highly variable, severe disorder of asymmetric and disproportionate overgrowth of body parts, connective tissue nevi, epidermal nevi, dysregulated adipose tissue, and vascular malformations. Many features of Proteus syndrome overlap with other overgrowth syndromes. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Cowden syndrome 6 (CWS6) [MIM:615109]: A form of Cowden syndrome, a hamartomatous polyposis syndrome with age-related penetrance. Cowden syndrome is characterized by hamartomatous lesions affecting derivatives of ectodermal, mesodermal and endodermal layers, macrocephaly, facial trichilemmomas (benign tumors of the hair follicle infundibulum), acral keratoses, papillomatous papules, and elevated risk for development of several types of malignancy, particularly breast carcinoma in women and thyroid carcinoma in both men and women. Colon cancer and renal cell carcinoma have also been reported. Hamartomas can be found in virtually every organ, but most commonly in the skin, gastrointestinal tract, breast and thyroid. Note: The disease is caused by mutations affecting the gene represented in this entry.

Research area

All research areas>Kinases and Phosphatases>Akt
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Akt1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human lung tissue

1: Akt1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung tissue

1: Akt1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach tissue

1: Akt1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach tissue

1: Akt1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: Akt1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: Akt1 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: Akt1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: Akt1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human appendix tissue

1: Akt1 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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