Rabbit Polyclonal AMPKalpha1/2 antibody (STJ91581)

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Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal AMPKalpha1/2 antibody (STJ91581)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Recommended protocols: check protocols

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Alternative names:

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PRKAA1 antibody, AMPK1 antibody,|5 AMP activated protein kinase alpha 1catalytic subunit antibody|5 AMP activated protein kinase catalytic alpha 1 chain antibody|5′ AMP activated protein kinase catalytic subunit alpha 1 antibody|5′-AMP-activated protein kinase catalytic subunit alpha-1 antibody|AAPK1 antibody|AAPK1_HUMAN antibody|ACACA kinase antibody|acetyl CoA carboxylase kinase antibody|AI194361 antibody|AI450832 antibody|AL024255 antibody|AMP -activate kinase alpha 1 subunit antibody|AMP-activated protein kinase, catalytic, alpha -1 antibody|AMPK 1 antibody|AMPK alpha 1 antibody|AMPK alpha 1 chain antibody|AMPK antibody|AMPK subunit alpha-1 antibody|AMPK1 antibody|AMPKa1 antibody|AMPKalpha1 antibody|C130083N04Rik antibody|cb116 antibody|EC 2.7.11.1 antibody|HMG CoA reductase kinase antibody|HMGCR kinase antibody|hormone sensitive lipase kinase antibody|Hydroxymethylglutaryl CoA reductase kinase antibody|im:7154392 antibody|kinase AMPK alpha1 antibody|MGC33776 antibody|MGC57364 antibody|OTTHUMP00000161795 antibody|OTTHUMP00000161796 antibody|PRKAA 1 antibody|PRKAA1 antibody|Protein kinase AMP activated alpha 1 catalytic subunit antibody|SNF1-like protein AMPK antibody|SNF1A antibody|Tau protein kinase PRKAA1 antibody|wu:fa94c10 antibody|Anti-AMPK alpha 1 antibody [Y365] (ab32047)
SCBT cat No: sc-130394|sc-19128|sc-19126|sc-398861|sc-74461|sc-25792|sc-19129|sc-19131|sc-19133|

Name

AMPKalpha1/2 Polyclonal Antibody

Catalogue No.

STJ91581

Reactivity

Human, Mouse, Rat, Monkey

Specificity

AMPKalpha1/2 Polyclonal Antibody detects endogenous levels of AMPKalpha1/2 protein.

Immunogen

Synthesized peptide derived from AMPKalpha1/2 at AA range 110-190

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

63 kDa

Formulation

AMPKalpha1/2 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

AMPKalpha1/2 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

5′-AMP-activated protein kinase catalytic subunit alpha-1 antibody, AMPK subunit alpha-1 antibody, Acetyl-CoA carboxylase kinase antibody, ACACA kinase antibody, Hydroxymethylglutaryl-CoA reductase kinase antibody, HMGCR kinase antibody, Tau-protein kinase PRKAA1 antibody,

Database links

Human UniProt/Swiss-Prot:Q13131 ;Human Entrez Gene: 5562;

Protein names

5′-AMP-activated protein kinase catalytic subunit alpha-1 , AMPK subunit alpha-1 , Acetyl-CoA carboxylase kinase , ACACA kinase , Hydroxymethylglutaryl-CoA reductase kinase , HMGCR kinase , Tau-protein kinase PRKAA1 ,

Protein function

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates ‘Ser-36’ of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. / ATP + a protein = ADP + a phosphoprotein. / ATP + [acetyl-CoA carboxylase] = ADP + [acetyl-CoA carboxylase] phosphate. / ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate. / ATP + [tau protein] = ADP + [tau protein] phosphate. / Mg2+ / Activated by phosphorylation on Thr-183. Binding of AMP to non-catalytic gamma subunit (PRKAG1, PRKAG2 or PRKAG3) results in allosteric activation, inducing phosphorylation on Thr-183. AMP-binding to gamma subunit also sustains activity by preventing dephosphorylation of Thr-183. ADP also stimulates Thr-183 phosphorylation, without stimulating already phosphorylated AMPK. ATP promotes dephosphorylation of Thr-183, rendering the enzyme inactive. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo . Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol.

Protein sequence and domain

The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity. / Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Ubiquitinated. / Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>AMPK
(View all antibody categories related to Kinases and Phosphatases)

Note

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Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: AMPKalpha1/2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: AMPKalpha1/2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon tissue

1: AMPKalpha1/2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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