Rabbit Polyclonal Anti-ASIC1 antibody (STJ98706)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Anti-ASIC1 antibody (STJ98706)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000, IHC 1:50-300; ELISA 1:5000-20000 1:10000-20000

Recommended protocols: check protocols

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SCBT cat No:

Name

Anti-ASIC1 antibody

Catalogue No.

STJ98706

Reactivity

Human, Mouse, Rat

Specificity

Anti-ASIC1 antibody detects endogenous ASIC1.

Immunogen

Synthetic peptide from human protein at AA range: 220-280.

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000, IHC 1:50-300; ELISA 1:5000-20000 1:10000-20000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

55kDa

Formulation

Anti-ASIC1 antibody was tube-contained in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Concentration

1 mg/ml

Purification

Anti-ASIC1 antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Database links

UniProt/Swiss-Prot:P78348

Protein function

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. .; Isoform 1 does not display proton-gated cation channel activity. .

Protein tissue specificity

Expressed in most or all neurons.

Protein sequence and domain

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC1 subfamily. Channel opening involves a conformation change that affects primarily the extracellular domain and the second transmembrane helix and its orientation in the membrane. In the open state, the second transmembrane helix is nearly perpendicular to the plane of the membrane; in the desensitized state it is strongly tilted. Besides, the second transmembrane domain is discontinuously helical in the open state. The GAS motif of the selectivity filter is in an extended conformation, giving rise to a distinct kink in the polypeptide chain. A domain swap between subunits gives rise to a full-length transmembrane helix (By similarity).

Protein post-translational modifications

Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. .

Protein cellular localization

Cell membrane ; Multi-pass membrane protein . Note=Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity). .

Note

AntibodyPlus can customize Anti-ASIC1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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