Rabbit Polyclonal Anti-GCK/GLK antibody (STJ99334)

$99.00$319.00

Reactivity: Human, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Anti-GCK/GLK antibody (STJ99334)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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|B lymphocyte serine/threonine protein kinase antibody|B lymphocyte serine/threonine-protein kinase antibody|BL44 antibody|GC kinase antibody|GCK antibody|Germinal center kinase antibody|germinal centre kinase (GC kinase) antibody|M4K2_HUMAN antibody|Map4k2 antibody|MAPK/ERK kinase kinase kinase 2 antibody|MEK kinase kinase 2 antibody|MEKKK 2 antibody|Mitogen activated protein kinase kinase kinase kinase 2 antibody|Mitogen-activated protein kinase kinase kinase kinase 2 antibody|Rab8 interacting protein antibody|Rab8 interacting protein, formerly antibody|Rab8-interacting protein antibody|RAB8IP antibody|RAB8IP, formerly antibody|Anti-Germinal Center Kinase antibody [EPR14304] (ab184169)
SCBT cat No: sc-398967|sc-6161|

Size

100ul

Name

Anti-GCK/GLK antibody

Catalogue No.

STJ99334

Reactivity

Human, Rat

Specificity

Anti-GCK/GLK antibody detects endogenous levels of GCK/GLK.

Immunogen

Synthesized peptide derived from human GCK/GLK. at AA range: 221-270.

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:10000-20000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

100kDa

Formulation

Anti-GCK/GLK antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-GCK/GLK antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

B lymphocyte serine/threonine protein kinase antibody, B lymphocyte serine/threonine-protein kinase antibody, BL44 antibody, GC kinase antibody, GCK antibody, Germinal center kinase antibody, germinal centre kinase (GC kinase) antibody, M4K2_HUMAN antibody, Map4k2 antibody, MAPK/ERK kinase kinase kinase 2 antibody, MEK kinase kinase 2 antibody, MEKKK 2 antibody, Mitogen activated protein kinase kinase kinase kinase 2 antibody, Mitogen-activated protein kinase kinase kinase kinase 2 antibody, Rab8 interacting protein antibody, Rab8 interacting protein, formerly antibody, Rab8-interacting protein antibody, RAB8IP antibody, RAB8IP, formerly antibody

Database links

UniProt/Swiss-Prot:Q12851

Protein function

Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. .

Protein tissue specificity

Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. .

Protein sequence and domain

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. ; Contains 1 CNH domain. ; Contains 1 protein kinase domain. The PEST domains are Pro-, Glu-, Ser-, and Thr-rich domains. Proteins with PEST domains are frequently targets of degradation by the ubiquitin proteasome.

Protein post-translational modifications

Polyubiquitinated through ‘Lys-48’-polyubiquitin chains, allowing proteasomal turnover. Ubiquitination requires the kinase activity of MAP4K2/GCK. .; Autophosphorylated in response to tumor necrosis factor (TNF), endotoxins or proinflammatory stimuli. Autophosphorylation leads to activation.

Protein cellular localization

Cytoplasm . Basolateral cell membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein .

Note

AntibodyPlus can customize Anti-GCK/GLK antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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