Rabbit Polyclonal Anti-IL-27A antibody (STJ98795)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC-P, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Anti-IL-27A antibody (STJ98795)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC-P, ELISA

Recommended dilution: IHC-P 1:50-300; ELISA 1:5000-20000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

|EBI3 antibody|IL 27 antibody|IL 27 p28 subunit antibody|IL 27A antibody|IL 30 antibody|IL-27 subunit alpha antibody|IL-27-A antibody|IL27 antibody|IL27-A antibody|IL27A antibody|IL27A_HUMAN antibody|IL27p28 antibody|IL30 antibody|Interleukin 27 antibody|Interleukin 27 subunit alpha antibody|Interleukin 27, 28-KD subunit antibody|Interleukin 30 antibody|Interleukin-27 subunit alpha antibody|p28 antibody|Anti-IL27 antibody – Aminoterminal end (ab62501)
SCBT cat No: sc-323971|sc-366222|

Name

Anti-IL-27A antibody

Catalogue No.

STJ98795

Reactivity

Human, Mouse, Rat

Specificity

Anti-IL-27A antibody detects endogenous IL-27A.

Immunogen

Synthetic peptide from human protein at AA range: 1-50.

Host

Rabbit

Applications

IHC-P, ELISA

Recommended dilution

IHC-P 1:50-300; ELISA 1:5000-20000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

Anti-IL-27A antibody was tube-contained in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Concentration

1 mg/ml

Purification

Anti-IL-27A antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

EBI3 antibody, IL 27 antibody, IL 27 p28 subunit antibody, IL 27A antibody, IL 30 antibody, IL-27 subunit alpha antibody, IL-27-A antibody, IL27 antibody, IL27-A antibody, IL27A antibody, IL27A_HUMAN antibody, IL27p28 antibody, IL30 antibody, Interleukin 27 antibody, Interleukin 27 subunit alpha antibody, Interleukin 27, 28-KD subunit antibody, Interleukin 30 antibody, Interleukin-27 subunit alpha antibody, p28 antibody

Database links

UniProt/Swiss-Prot:Q8NEV9

Protein function

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication. .

Protein tissue specificity

Expressed in monocytes and in placenta. .

Protein sequence and domain

Belongs to the IL-6 superfamily.

Protein post-translational modifications

O-glycosylated. .

Protein cellular localization

Secreted . Note=Does not seem to be secreted without coexpression of EBI3.

Note

AntibodyPlus can customize Anti-IL-27A antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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