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Rabbit Polyclonal Anti-IRF3 antibody (STJ98960)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-IRF3 antibody.
Check alternative names for the antibodyExpand
|Interferon regulatory factor 3 antibody|IRF 3 antibody|IRF-3 antibody|IRF3 antibody|IRF3_HUMAN antibody|MGC94729 antibody|Anti-IRF3 antibody [EPR2418Y] (ab68481)
SCBT cat No: sc-15991|sc-376455|sc-9082|sc-33641|sc-33642|
|Catalogue No.|| |
Anti-IRF3 antibody detects endogenous IRF3.
Synthetic peptide from human protein at AA range: 279-352.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
Anti-IRF3 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-IRF3 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Interferon regulatory factor 3 antibody, IRF 3 antibody, IRF-3 antibody, IRF3 antibody, IRF3_HUMAN antibody, MGC94729 antibody
|Database links|| |
|Protein function|| |
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
|Protein tissue specificity|| |
Expressed constitutively in a variety of tissues.
|Involvement in disease|| |
Herpes simplex encephalitis 7 (HSE7) [MIM:616532]: A rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. . Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the IRF family. ; Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
|Protein post-translational modifications|| |
Constitutively phosphorylated on many Ser/Thr residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Phosphorylated at Ser-396 by IKBKE upon ssRNA viral infection. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. Phosphorylation at Ser-386 by TBK1 results in oligomerization. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. .; (Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3. .; Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation. .; ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. .
|Protein cellular localization|| |
Cytoplasm . Nucleus . Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
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St John’s Laboratory Ltd.
|Product type|| |
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