Rabbit Polyclonal Anti-PRC1 antibody (STJ98681)

$99.00$319.00

Reactivity: Human
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Anti-PRC1 antibody (STJ98681)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:5000-20000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Anti-PRC1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

|Anaphase spindle elongation 1 homolog antibody|ASE1 antibody|PRC1 antibody|PRC1_HUMAN antibody|Protein regulating cytokinesis 1 antibody|Protein regulator of cytokinesis 1 antibody|Anti-PRC1 antibody [EP1513Y] (ab51248)
SCBT cat No: sc-377544|sc-11768|sc-16795|sc-12891|sc-130604|sc-377573|

Name

Anti-PRC1 antibody

Catalogue No.

STJ98681

Reactivity

Human

Specificity

Anti-PRC1 antibody detects endogenous PRC1.

Immunogen

Synthetic peptide from human protein at AA range: 460-520.

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:5000-20000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

72kDa

Formulation

Anti-PRC1 antibody was tube-contained in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Concentration

1 mg/ml

Purification

Anti-PRC1 antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Anaphase spindle elongation 1 homolog antibody, ASE1 antibody, PRC1 antibody, PRC1_HUMAN antibody, Protein regulating cytokinesis 1 antibody, Protein regulator of cytokinesis 1 antibody

Database links

UniProt/Swiss-Prot:O43663

Protein function

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436). .

Protein tissue specificity

Overexpressed in bladder cancer cells (PubMed:17409436). .

Protein sequence and domain

Belongs to the MAP65/ASE1 family. Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.

Protein post-translational modifications

Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. Phosphorylation by CDK1 prevents PLK1-binding: upon degradation of CDK1 at anaphase and dephosphorylation, it is then phosphorylated by PLK1, leading to cytokinesis. .

Protein cellular localization

Nucleus . Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Midbody . Note=Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436). Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. .

Note

AntibodyPlus can customize Anti-PRC1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!