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Rabbit Polyclonal Anti-Rb (Acetyl K873/K874) antibody (STJ98847)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:5000-20017
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
|AF6q21 antibody|AF6q21 protein antibody|DKFZp781A0677 antibody|FKHR2 antibody|FKHRL 1 antibody|FKHRL1 antibody|FKHRL1P2 antibody|Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody|Forkhead box O3 antibody|Forkhead box O3A antibody|Forkhead box protein O3 antibody|Forkhead box protein O3A antibody|Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody|Forkhead homolog (rhabdomyosarcoma) like 1 antibody|Forkhead in rhabdomyosarcoma like 1 antibody|Forkhead in rhabdomyosarcoma-like 1 antibody|FOX O3A antibody|FOXO2 antibody|foxo3 antibody|FOXO3_HUMAN antibody|FOXO3A antibody|MGC12739 antibody|MGC31925 antibody|Anti-FOXO3A antibody – ChIP Grade (ab12162)
SCBT cat No: sc-34894|sc-34895|sc-48348|sc-11351|sc-34897|sc-9813|sc-9812|sc-48503|sc-374642|sc-48506|
Anti-Rb (Acetyl K873/K874) antibody
|Catalogue No.|| |
Human, Mouse, Rat
Anti-Rb (Acetyl K873/K874) antibody detects endogenous levels of FoxO3A.
Synthesized peptide derived from Human FoxO3A at AA range: 550-580.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:5000-20017
Anti-Rb (Acetyl K873/K874) antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-Rb (Acetyl K873/K874) antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
AF6q21 antibody, AF6q21 protein antibody, DKFZp781A0677 antibody, FKHR2 antibody, FKHRL 1 antibody, FKHRL1 antibody, FKHRL1P2 antibody, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody, Forkhead box O3 antibody, Forkhead box O3A antibody, Forkhead box protein O3 antibody, Forkhead box protein O3A antibody, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody, Forkhead homolog (rhabdomyosarcoma) like 1 antibody, Forkhead in rhabdomyosarcoma like 1 antibody, Forkhead in rhabdomyosarcoma-like 1 antibody, FOX O3A antibody, FOXO2 antibody, foxo3 antibody, FOXO3_HUMAN antibody, FOXO3A antibody, MGC12739 antibody, MGC31925 antibody
|Database links|| |
|Protein function|| |
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation. .
|Protein tissue specificity|| |
|Involvement in disease|| |
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with KMT2A/MLL1.
|Protein sequence and domain|| |
Contains 1 fork-head DNA-binding domain.
|Protein post-translational modifications|| |
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn’t appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4/MST1 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. Phosphorylation by AMPK leads to the activation of transcriptional activity without affecting subcellular localization. Phosphorylation by MAPKAPK5 promotes nuclear localization and DNA-binding, leading to induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation (PubMed:10102273, PubMed:11154281, PubMed:16751106, PubMed:17711846, PubMed:18593906, PubMed:21329882). Phosphorylated by CHUK/IKKA and IKBKB/IKKB. TNF-induced inactivation of FOXO3 requires its phosphorylation at Ser-644 by IKBKB/IKKB which promotes FOXO3 retention in the cytoplasm, polyubiquitination and ubiquitin-mediated proteasomal degradation (PubMed:15084260). May be dephosphorylated by calcineurin A on Ser-299 which abolishes FOXO3 transcriptional activity (By similarity). .; Deacetylation by SIRT1 or SIRT2 stimulates interaction of FOXO3 with SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation (PubMed:21841822). Deacetylation by SIRT2 stimulates FOXO3-mediated transcriptional activity in response to oxidative stress (By similarity). .; Heavily methylated by SET9 which decreases stability, while moderately increasing transcriptional activity. The main methylation site is Lys-271. Methylation doesn’t affect subcellular location. .; Polyubiquitinated. Ubiquitinated by a SCF complex containing SKP2, leading to proteasomal degradation. .
|Protein cellular localization|| |
Cytoplasm, cytosol . Nucleus . Note=Retention in the cytoplasm contributes to its inactivation. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. .
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St John’s Laboratory Ltd.
|Product type|| |
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