Rabbit Polyclonal Anti-Rb (Acetyl K873/K874) antibody (STJ98847)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Anti-Rb (Acetyl K873/K874) antibody (STJ98847)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-2000; ELISA 1:5000-20017

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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|AF6q21 antibody|AF6q21 protein antibody|DKFZp781A0677 antibody|FKHR2 antibody|FKHRL 1 antibody|FKHRL1 antibody|FKHRL1P2 antibody|Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody|Forkhead box O3 antibody|Forkhead box O3A antibody|Forkhead box protein O3 antibody|Forkhead box protein O3A antibody|Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody|Forkhead homolog (rhabdomyosarcoma) like 1 antibody|Forkhead in rhabdomyosarcoma like 1 antibody|Forkhead in rhabdomyosarcoma-like 1 antibody|FOX O3A antibody|FOXO2 antibody|foxo3 antibody|FOXO3_HUMAN antibody|FOXO3A antibody|MGC12739 antibody|MGC31925 antibody|Anti-FOXO3A antibody – ChIP Grade (ab12162)
SCBT cat No: sc-34894|sc-34895|sc-48348|sc-11351|sc-34897|sc-9813|sc-9812|sc-48503|sc-374642|sc-48506|

Name

Anti-Rb (Acetyl K873/K874) antibody

Catalogue No.

STJ98847

Reactivity

Human, Mouse, Rat

Specificity

Anti-Rb (Acetyl K873/K874) antibody detects endogenous levels of FoxO3A.

Immunogen

Synthesized peptide derived from Human FoxO3A at AA range: 550-580.

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-2000; ELISA 1:5000-20017

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

Anti-Rb (Acetyl K873/K874) antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Anti-Rb (Acetyl K873/K874) antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

AF6q21 antibody, AF6q21 protein antibody, DKFZp781A0677 antibody, FKHR2 antibody, FKHRL 1 antibody, FKHRL1 antibody, FKHRL1P2 antibody, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody, Forkhead box O3 antibody, Forkhead box O3A antibody, Forkhead box protein O3 antibody, Forkhead box protein O3A antibody, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody, Forkhead homolog (rhabdomyosarcoma) like 1 antibody, Forkhead in rhabdomyosarcoma like 1 antibody, Forkhead in rhabdomyosarcoma-like 1 antibody, FOX O3A antibody, FOXO2 antibody, foxo3 antibody, FOXO3_HUMAN antibody, FOXO3A antibody, MGC12739 antibody, MGC31925 antibody

Database links

UniProt/Swiss-Prot:O43524

Protein function

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation. .

Protein tissue specificity

Ubiquitous. .

Involvement in disease

Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with KMT2A/MLL1.

Protein sequence and domain

Contains 1 fork-head DNA-binding domain.

Protein post-translational modifications

In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn’t appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4/MST1 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. Phosphorylation by AMPK leads to the activation of transcriptional activity without affecting subcellular localization. Phosphorylation by MAPKAPK5 promotes nuclear localization and DNA-binding, leading to induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation (PubMed:10102273, PubMed:11154281, PubMed:16751106, PubMed:17711846, PubMed:18593906, PubMed:21329882). Phosphorylated by CHUK/IKKA and IKBKB/IKKB. TNF-induced inactivation of FOXO3 requires its phosphorylation at Ser-644 by IKBKB/IKKB which promotes FOXO3 retention in the cytoplasm, polyubiquitination and ubiquitin-mediated proteasomal degradation (PubMed:15084260). May be dephosphorylated by calcineurin A on Ser-299 which abolishes FOXO3 transcriptional activity (By similarity). .; Deacetylation by SIRT1 or SIRT2 stimulates interaction of FOXO3 with SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation (PubMed:21841822). Deacetylation by SIRT2 stimulates FOXO3-mediated transcriptional activity in response to oxidative stress (By similarity). .; Heavily methylated by SET9 which decreases stability, while moderately increasing transcriptional activity. The main methylation site is Lys-271. Methylation doesn’t affect subcellular location. .; Polyubiquitinated. Ubiquitinated by a SCF complex containing SKP2, leading to proteasomal degradation. .

Protein cellular localization

Cytoplasm, cytosol . Nucleus . Note=Retention in the cytoplasm contributes to its inactivation. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. .

Research area

All research areas>Transcription Regulators>FKHR
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Anti-Rb (Acetyl K873/K874) antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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