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Rabbit Polyclonal Anti-Smad1/5/9 antibody (STJ99327)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-2000; ELISA 1:10000-20000
Recommended protocols: check protocols
Click or hover above images to see image description for Anti-Smad1/5/9 antibody.
Check alternative names for the antibodyExpand
|MAD mothers against decapentaplegic homolog 1 antibody|MAD mothers against decapentaplegic homolog 5 antibody|MADH1 antibody|MADH5 antibody|Mothers against DPP homolog 1 antibody|Mothers against DPP homolog 5 antibody|SMAD family member 1 antibody|SMAD family member 5 antibody|SMAD1 antibody|SMAD5 antibody|Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273)
SCBT cat No: sc-12353|sc-101801|sc-11769|sc-130218|sc-514317|sc-165305|sc-515132|
|Catalogue No.|| |
Human, Mouse, Rat
Anti-Smad1/5/9 antibody detects endogenous levels of Smad1/5/9.
Synthesized peptide derived from human Smad1/5/9. at AA range: 291-340.
|Recommended dilution|| |
WB 1:500-2000; ELISA 1:10000-20000
|Molecular weight|| |
Anti-Smad1/5/9 antibody was tube-contained in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Anti-Smad1/5/9 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
MAD mothers against decapentaplegic homolog 1 antibody, MAD mothers against decapentaplegic homolog 5 antibody, MADH1 antibody, MADH5 antibody, Mothers against DPP homolog 1 antibody, Mothers against DPP homolog 5 antibody, SMAD family member 1 antibody, SMAD family member 5 antibody, SMAD1 antibody, SMAD5 antibody
|Database links|| |
|Protein function|| |
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. .
|Protein tissue specificity|| |
Ubiquitous. Highest expression seen in the heart and skeletal muscle.
|Involvement in disease|| |
Note=SMAD1 variants may be associated with susceptibility to pulmonary hypertension, a disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial pulmonary hypertension is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs. .
|Protein sequence and domain|| |
Belongs to the dwarfin/SMAD family. ; Contains 1 MH1 (MAD homology 1) domain. ; Contains 1 MH2 (MAD homology 2) domain. The MH2 domain mediates phosphorylation-dependent trimerization through L3 loop binding of phosphoserines in the adjacent subunit.
|Protein post-translational modifications|| |
Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. .; Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. .
|Protein cellular localization|| |
Cytoplasm . Nucleus . Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). .
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St John’s Laboratory Ltd.
|Product type|| |
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