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Rabbit Polyclonal Anti-ZAP-70 antibody (STJ98801)
Supplier: St John’s Laboratory Ltd.
Recommended applications: IHC-P, ELISA
Recommended dilution: IHC-P 1:50-300; ELISA 1:5000-20000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
|70 kDa zeta associated protein antibody|Truncated ZAP kinase antibody|Tyrosine protein kinase ZAP70 antibody|Tyrosine-protein kinase ZAP-70 antibody|TZK antibody|ZAP 70 antibody|ZAP70 antibody|ZAP70_HUMAN antibody|Zeta chain associated protein kinase 70kD antibody|Zeta chain associated protein kinase 70kDa antibody|Zeta chain associated protein kinase 70kDa isoform 1 antibody|Zeta chain associated protein kinase 70kDa isoform 2 antibody|Zeta chain TCR associated protein kinase 70kD antibody|Zeta chain TCR associated protein kinase 70kDa antibody|Anti-ZAP70 antibody [YE291] (ab32429)
SCBT cat No: sc-12945|sc-101822|sc-12946|sc-33526|sc-23889|sc-26168|sc-71948|sc-52552|sc-50478|sc-100809|sc-376218|sc-98349|sc-101547|sc-390535|sc-55266|
|Catalogue No.|| |
Anti-ZAP-70 antibody detects endogenous ZAP-70.
Synthetic peptide from human protein at AA range: 391-440.
|Recommended dilution|| |
IHC-P 1:50-300; ELISA 1:5000-20000
Anti-ZAP-70 antibody was tube-contained in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Anti-ZAP-70 antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
70 kDa zeta associated protein antibody, Truncated ZAP kinase antibody, Tyrosine protein kinase ZAP70 antibody, Tyrosine-protein kinase ZAP-70 antibody, TZK antibody, ZAP 70 antibody, ZAP70 antibody, ZAP70_HUMAN antibody, Zeta chain associated protein kinase 70kD antibody, Zeta chain associated protein kinase 70kDa antibody, Zeta chain associated protein kinase 70kDa isoform 1 antibody, Zeta chain associated protein kinase 70kDa isoform 2 antibody, Zeta chain TCR associated protein kinase 70kD antibody, Zeta chain TCR associated protein kinase 70kDa antibody
|Database links|| |
|Protein function|| |
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR). .
|Protein tissue specificity|| |
Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells. .
|Involvement in disease|| |
Immunodeficiency 48 (IMD48) [MIM:269840]: A form of severe immunodeficiency characterized by a selective absence of CD8+ T-cells. . Note=The disease is caused by mutations affecting the gene represented in this entry.; Autoimmune disease, multisystem, infantile-onset, 2 (ADMIO2) [MIM:617006]: An autosomal recessive, autoimmune disorder characterized by systemic manifestations including blistering skin disease, uncontrollable bullous pemphigoid, inflammatory colitis, autoimmune hypothyroidism, proteinuria and nephrotic syndrome. . Note=The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. ; Contains 1 protein kinase domain. ; Contains 2 SH2 domains. Composed of 2 N-terminal SH2 domains and a C-terminal kinase domain. The tandem SH2 domains bind to the doubly phosphorylated tyrosine-based activation motif (ITAM) of CD247/CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH (By similarity). The interdomain B located between the second SH2 and the kinase domain contains 3 tyrosines (Tyr-292, Tyr-315, Tyr-319) that are phosphorylated following TCR activation. These sites have been implicated in binding to other signaling molecules including CBL or VAV1. Thus, ZAP70 can also function as a scaffold by recruiting additional factors to the stimulated TCR complex.
|Protein post-translational modifications|| |
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22. .
|Protein cellular localization|| |
Cytoplasm . Cell membrane ; Peripheral membrane protein . Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). .
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St John’s Laboratory Ltd.
|Product type|| |
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