Rabbit polyclonal ANXA1 antibody (A1118)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ANXA1 antibody (A1118)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ANXA1 antibody,ANX1 antibody,LPC1 antibody
Annexin 1 antibody|Annexin A1 antibody|Annexin I (lipocortin I) antibody|Annexin I antibody|Annexin-1 antibody|AnnexinA1 antibody|AnnexinI antibody|ANX 1 antibody|ANX A1 antibody|ANX1 antibody|ANXA 1 antibody|ANXA1 antibody|ANXA1 protein antibody|ANXA1_HUMAN antibody|Calpactin 2 antibody|Calpactin II antibody|Calpactin-2 antibody|CalpactinII antibody|Chromobindin 9 antibody|Chromobindin-9 antibody|Chromobindin9 antibody|HGNC:533 antibody|Lipocortin 1 antibody|Lipocortin I antibody|Lipocortin1 antibody|LipocortinI antibody|LPC 1 antibody|LPC1 antibody|p35 antibody|Phospholipase A2 inhibitory protein antibody|Anti-Annexin A1 antibody [EPR19342] (ab214486)

SCBT cat No: sc-130305|sc-65872|sc-53158|sc-53159|sc-12740|sc-11387|sc-1923|sc-47696|sc-30757|

Name

Rabbit polyclonal ANXA1 antibody

Catalogue No.

A1118

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ANXA1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 39kDa/Observed: Refer to Figures

Formulation

ANXA1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ANXA1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ANXA1 antibody,ANX1 antibody,LPC1 antibody

Database links

Human UniProt/Swiss-Prot:P04083

Protein names

ANXA1,ANX1,LPC1

Protein function

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity . Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing . Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades . Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors . Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells . Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells . Has no effect on unstimulated T cells . Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration . Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton . Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes . Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).

Protein tissue specificity

Detected in resting neutrophils . Detected in peripheral blood T-cells . Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors . Detected in placenta (at protein level) . Detected in liver.

Protein sequence and domain

The full-length protein can bind eight Ca(2+) ions via the annexin repeats. Calcium binding causes a major conformation change that modifies dimer contacts and leads to surface exposure of the N-terminal phosphorylation sites; in the absence of Ca(2+), these sites are buried in the interior of the protein core. The N-terminal region becomes disordered in response to calcium-binding.; The N-terminal 26 amino acids are sufficient for its extracellular functions in the regulation of inflammation and wound healing . Acylated peptides that contain the first 26 amino acids of the mature protein can activate signaling via the formyl peptide receptors .Belongs to the annexin family.; Contains 4 annexin repeats.

Protein post-translational modifications

Phosphorylated by protein kinase C, EGFR and TRPM7 . Phosphorylated in response to EGF treatment .; Sumoylated.

Protein cellular localization

Nucleus . Cytoplasm . Cell projection, cilium . Cell membrane . Membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Basolateral cell membrane . Apical cell membrane . Lateral cell membrane . Secreted . Secreted, extracellular space . Cell membrane ; Peripheral membrane protein ; Extracellular side . Secreted, exosome . Cytoplasmic vesicle, secretory vesicle lumen . Cell projection, phagocytic cup . Early endosome . Cytoplasmic vesicle membrane ; Peripheral membrane protein . Note: Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles . Detected in gelatinase granules in resting neutrophils . Secretion is increased in response to wounding and inflammation . Secretion is increased upon T-cell activation . Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect . Colocalizes with actin fibers at phagocytic cups (By similarity). Displays calcium-dependent binding to phospholipid membranes .

Background

The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1). Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4). Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6). Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7). Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10). Annexin A1 has also been identified as one of the ‘eat-me’ signals on apoptotic cells that are to be recognized and ingested by phagocytes (11). Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14).

Research area

All research areas>Signaling Intermediates>Annexin
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize ANXA1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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