Rabbit polyclonal AP2A1 antibody (A6863)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal AP2A1 antibody (A6863)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IP 1:20 – 1:50

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ADTAA antibody, CLAPA1 antibody, AP2-ALPHA antibody
100 kDa coated vesicle protein A antibody|ADTAA antibody|ADTAB antibody|Alpha adaptin A antibody|Alpha adaptin C antibody|Alpha adaptin C; Huntingtin interacting protein J antibody|Alpha1 adaptin antibody|Alpha2 adaptin antibody|AP 2 complex subunit alpha 1 antibody|AP 2 complex subunit alpha 2 antibody|AP2 alpha antibody|AP2A1 antibody|AP2A2 antibody|CLAPA1 antibody|CLAPA2 antibody|Clathrin assembly protein complex 2 alpha A large chain antibody|Clathrin assembly protein complex 2 alpha C large chain antibody|Clathrin associated/assembly/adaptor protein large alpha 1 antibody|Clathrin associated/assembly/adaptor protein large alpha 2 antibody|HIP 9 antibody|HIP9 antibody|Huntingtin interacting protein antibody|Huntingtin interacting protein HYPJ antibody|Huntingtin interacting protein J antibody|Huntingtin yeast partner J antibody|HYPJ antibody|Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody|Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody|Anti-alpha Adaptin antibody [AP6] (ab2730)

SCBT cat No: sc-377376|

Name

Rabbit polyclonal AP2A1 antibody

Catalogue No.

A6863

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human AP2A1

Host

Rabbit

Applications

WB, IHC, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IP 1:20 – 1:50

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 108kDa/Observed: Refer to figures

Formulation

AP2A1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

AP2A1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ADTAA antibody, CLAPA1 antibody, AP2-ALPHA antibody

Database links

Human UniProt/Swiss-Prot:O95782

Protein names

ADTAA, CLAPA1, AP2-ALPHA

Protein function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).

Protein tissue specificity

Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.

Protein sequence and domain

Belongs to the adaptor complexes large subunit family.

Protein cellular localization

Cell membrane . Membrane, coated pit ; Peripheral membrane protein ; Cytoplasmic side . Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.

Background

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

Note

AntibodyPlus can customize AP2A1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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