Rabbit polyclonal AP2B1 antibody (A1995)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal AP2B1 antibody (A1995)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal AP2B1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

ADTB2 antibody, AP105B antibody, CLAPB1 antibody, AP2-BETA antibody
Adaptin, beta 2 (beta) antibody|ADTB2 antibody|AP-2 complex subunit beta antibody|AP105B antibody|AP2 BETA antibody|Ap2b1 antibody|AP2B1_HUMAN antibody|Beta adaptin antibody|Beta-2-adaptin antibody|Beta-adaptin antibody|Beta2 adaptin antibody|CLAPB1 antibody|Clathrin assembly protein complex 2 beta large chain antibody|Clathrin associated/assembly/adaptor protein, large, beta 1 antibody|DKFZp781K0743 antibody|Plasma membrane adaptor HA2/AP2 adaptin beta subunit antibody|Anti-AP2B1 antibody (ab205014)

SCBT cat No: sc-130925|sc-134230|sc-162444|sc-162445|sc-80632|

Name

Rabbit polyclonal AP2B1 antibody

Catalogue No.

A1995

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human AP2B1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 105kDa/Observed: Refer to Figures

Formulation

AP2B1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

AP2B1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ADTB2 antibody, AP105B antibody, CLAPB1 antibody, AP2-BETA antibody

Database links

Human UniProt/Swiss-Prot:P63010

Protein names

ADTB2, AP105B, CLAPB1, AP2-BETA

Protein function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.

Protein sequence and domain

Belongs to the adaptor complexes large subunit family.

Protein post-translational modifications

Phosphorylation at Tyr-737 by SRC occurs at the plasma membrane in clathrin-coated vesicles (CCVs).

Protein cellular localization

Cell membrane . Membrane, coated pit ; Peripheral membrane protein ; Cytoplasmic side . Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.

Background

The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.

Note

AntibodyPlus can customize AP2B1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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