Rabbit polyclonal AP2M1 antibody (A2492)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal AP2M1 antibody (A2492)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal AP2M1 antibody.

Alternative names:

Check alternative names for the antibody

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AP50 antibody, CLAPM1 antibody, mu2 antibody
AP 2 mu 2 chain antibody|AP-2 complex subunit mu antibody|AP-2 mu chain antibody|Ap2m1 antibody|AP2M1_HUMAN antibody|AP50 antibody|CLAPM1 antibody|Clathrin adaptor complex AP2 mu subunit antibody|Clathrin assembly protein complex 2 medium chain antibody|Clathrin associated/assembly/adaptor protein medium 1 antibody|Clathrin coat adaptor protein AP50 antibody|Clathrin coat assembly protein AP50 antibody|Clathrin coat associated protein AP50 antibody|Clathrin coat-associated protein AP50 antibody|HA2 50 kDa subunit antibody|mu2 antibody|Plasma membrane adaptor AP-2 50 kDa protein antibody|Anti-AP2M1 antibody [EP2695Y] (ab75995)

SCBT cat No: sc-49150|sc-99026|sc-49151|

Name

Rabbit polyclonal AP2M1 antibody

Catalogue No.

A2492

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human AP2M1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 49kDa/Observed: Refer to Figures

Formulation

AP2M1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

AP2M1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

AP50 antibody, CLAPM1 antibody, mu2 antibody

Database links

Human UniProt/Swiss-Prot:Q96CW1

Protein names

AP50, CLAPM1, mu2

Protein function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at ‘Tyr-156’ in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).

Protein sequence and domain

Belongs to the adaptor complexes medium subunit family.; Contains 1 MHD (mu homology) domain.

Protein cellular localization

Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.

Background

The AP-2 coat assembly protein complex is an important component of clathrin-coated pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of alpha, beta, u, and sigma protein subunits. The 50 kDa u subunit (AP-2u, AP2M1) is located at the core of the AP-2 complex and mediates interaction between the cargo protein and the clathrin-coated pit (1-4). The carboxy-terminal AP2M1 region recognizes the tyrosine-based, endocytotic sorting motif YXXphi found in cargo proteins and helps to bring the cargo protein to the clathrin-coated pit. Non-canonical, tyrosine-based endocytotic sorting signals can also promote interaction between cargo proteins and AP2M1 (5,6). AP2M1 plays an essential role in molecular signaling as it couples receptor-mediated endocytosis and pathways involving membrane receptors (7-9), matrix metalloproteinases (10), and ion channel proteins (11). Phosphorylation of specific AP2M1 residues and binding of lipids to this adaptor protein can regulate AP2M1 activity (12,13). Phosphorylation of AP2M1 at Thr156 by adaptor-associated kinase 1 (AAK1) stimulates affinity binding of AP2M1 to cargo protein signals (14).

Research area

All research areas>Transport and Trafficking>Adaptin Related Adapter Protein
(View all antibody categories related to Transport and Trafficking)

Note

AntibodyPlus can customize AP2M1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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