Rabbit polyclonal ARHGEF7 antibody (A1108)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ARHGEF7 antibody (A1108)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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ARHGEF7 antibody,BETA-PIX antibody,COOL1 antibody,DKFZp686C12170 antibody,DKFZp761K1021 antibody,KIAA0142 antibody,KIAA0412 antibody,Nbla10314 antibody,P50 antibody,P50BP antibody,P85 antibody,P85COOL1 antibody,P85SPR antibody,PAK3 antibody,PIXB antibody
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SCBT cat No: sc-136035|sc-10932|sc-25418|sc-393184|sc-10931|

Name

Rabbit polyclonal ARHGEF7 antibody

Catalogue No.

A1108

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ARHGEF7

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 90kDa/Observed: Refer to Figures

Formulation

ARHGEF7 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ARHGEF7 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ARHGEF7 antibody,BETA-PIX antibody,COOL1 antibody,DKFZp686C12170 antibody,DKFZp761K1021 antibody,KIAA0142 antibody,KIAA0412 antibody,Nbla10314 antibody,P50 antibody,P50BP antibody,P85 antibody,P85COOL1 antibody,P85SPR antibody,PAK3 antibody,PIXB antibody

Database links

Human UniProt/Swiss-Prot:Q14155

Protein names

ARHGEF7,BETA-PIX,COOL1,DKFZp686C12170,DKFZp761K1021,KIAA0142,KIAA0412,Nbla10314,P50,P50BP,P85,P85COOL1,P85SPR,PAK3,PIXB

Protein function

Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.

Protein sequence and domain

Contains 1 CH (calponin-homology) domain.; Contains 1 DH (DBL-homology) domain.; Contains 1 PH domain.; Contains 1 SH3 domain.

Protein post-translational modifications

Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1 (By similarity). Phosphorylated on Ser-694 by CaMK1; enhancement of GEF activity and downstream activation of RAC1.

Protein cellular localization

Cell junction, focal adhesion . Cell projection, ruffle . Cytoplasm, cell cortex . Cell projection, lamellipodium . Note: Detected at cell adhesions. A small proportion is detected at focal adhesions.

Background

Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/alphapix and cool1/betaPix, the latter having two splice variants that vary in their carboxy termini (1). Cool1/betaPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization. Cool1/betaPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3,4). Phosphorylation of cool1/betaPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/betaPix, regulating formation of growth cones in response to growth factors (4). Growth factor induced activation of Rac1 via cool1/betaPix was later shown to occur independently of subcellular localization (5). Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/betaPix and activation of cdc42 (6).

Note

AntibodyPlus can customize ARHGEF7 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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