Rabbit Polyclonal ASC antibody (STJ91730)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal ASC antibody (STJ91730)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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PYCARD antibody, ASC antibody, CARD5 antibody, TMS1 antibody,|Apoptosis associated speck like protein containing a CARD antibody|Apoptosis-associated speck-like protein containing a CARD antibody|ASC antibody|ASC_HUMAN antibody|CARD 5 antibody|CARD5 antibody|Caspase recruitment domain containing protein 5 antibody|Caspase recruitment domain protein 5 antibody|Caspase recruitment domain-containing protein 5 antibody|hASC antibody|MGC10332 antibody|PYCARD antibody|PYD and CARD domain containing antibody|PYD and CARD domain containing protein antibody|PYD and CARD domain-containing protein antibody|Target of methylation induced silencing 1 antibody|Target of methylation-induced silencing 1 antibody|TMS 1 antibody|TMS antibody|TMS1 antibody|Anti-TMS1 antibody [2A7] (ab128097)
SCBT cat No: sc-514414|sc-33958|sc-514559|sc-271054|sc-30153|sc-22514|

Name

ASC Polyclonal Antibody

Catalogue No.

STJ91730

Reactivity

Human, Mouse, Rat

Specificity

ASC Polyclonal Antibody detects endogenous levels of ASC protein.

Immunogen

Synthesized peptide derived from ASC at AA range 10-90

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

21 kDa

Formulation

ASC Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

ASC Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Apoptosis-associated speck-like protein containing a CARD antibody, hASC antibody, Caspase recruitment domain-containing protein 5 antibody, PYD and CARD domain-containing protein antibody, Target of methylation-induced silencing 1 antibody

Database links

Human UniProt/Swiss-Prot:Q9ULZ3;Mouse UniPort/Swiss-Prot: Q9EPB4;Rat UniProt/Swiss-Port: Q8CHK8;Human Entrez Gene: 29108;Mouse Entrez Gene: 66824;Rat Entrez Gene: Rn.7817

Protein names

Apoptosis-associated speck-like protein containing a CARD , hASC , Caspase recruitment domain-containing protein 5 , PYD and CARD domain-containing protein , Target of methylation-induced silencing 1

Protein function

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing.

Protein tissue specificity

Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt’s lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.

Protein sequence and domain

The CARD domain mediates interaction with CASP1 and NLRC4 (PubMed:14634131 and PubMed:11967258). / The pyrin domain mediates homotypic interactions with pyrin domains of proteins such as of NLRP3, PYDC1, PYDC2 and AIM2. / Contains 1 CARD domain. / Contains 1 pyrin domain.

Protein post-translational modifications

Phosphorylated.

Protein cellular localization

Cytoplasm / Endoplasmic reticulum / Mitochondrion / Nucleus

Research area

All research areas>Tumor Suppressor/Apoptosis>ASC
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize ASC Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunohistochemical analysis of paraffin embedded Human liver tissue

1: ASC Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: ASC Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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