Rabbit polyclonal Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

$69.00$259.00

Reactivity: Human,Mouse,Rat,Wide range
Applications: WB,IHC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Asymmetric DiMethyl-Histone H4-R3 antibody.

Alternative names:

Check alternative names for the antibody

Expand

H4R3me2a antibody, H3t antibody, H3.4 antibody, H3/g antibody, H3FT antibody
H4_HUMAN antibody|H4F2 antibody|H4FN antibody|HIST1H4 antibody|HIST2H4 antibody|Hist4h4 antibody|HISTH4H4 antibody|Histone H4 antibody|methyl histone H4 antibody|methylated histone H4 antibody|Anti-Histone H4K20me1 Antibody – ChIP Grade (ab9051)

SCBT cat No:

Name

Rabbit polyclonal Asymmetric DiMethyl-Histone H4-R3 antibody

Catalogue No.

A2376

Reactivity

Human, Mouse, Rat, Wide range

Immunogen

A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4

Host

Rabbit

Applications

WB, IHC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200
IP 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 11kDa/Observed: Refer to Figures

Formulation

Asymmetric DiMethyl-Histone H4-R3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Asymmetric DiMethyl-Histone H4-R3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

H4R3me2a antibody, H3t antibody, H3.4 antibody, H3/g antibody, H3FT antibody

Database links

Human UniProt/Swiss-Prot:P62805

Protein names

H4R3me2a, H3t, H3.4, H3/g, H3FT

Protein function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Protein sequence and domain

Belongs to the histone H4 family.

Protein post-translational modifications

Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.; Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.; Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.; Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by KMT5B and KMT5C and induces gene silencing.; Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.; Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).; Sumoylated, which is associated with transcriptional repression.; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Protein cellular localization

Nucleus. Chromosome.

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.

Research area

All research areas>Transcription Regulators>Histone
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Asymmetric DiMethyl-Histone H4-R3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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