Rabbit polyclonal ATG3 antibody (A5809)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ATG3 antibody (A5809)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

APG3 antibody, APG3L antibody, PC3-96 antibody, APG3-LIKE antibody
2610016C12Rik antibody|DKFZp564M1178 antibody|FLJ22125 antibody|hApg3 antibody|MGC15201 antibody|OTTHUMP00000214547 antibody|OTTHUMP00000214548 antibody|PC3 96 antibody|Protein PC3-96 antibody|Ubiquitin-like-conjugating enzyme ATG3 antibody|Anti-Apg3 antibody [EPR4801] (ab108251)

SCBT cat No: sc-393660|sc-100508|sc-393623|sc-70139|

Name

Rabbit polyclonal ATG3 antibody

Catalogue No.

A5809

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ATG3

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 36kDa/Observed: Refer to Figures

Formulation

ATG3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ATG3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

APG3 antibody, APG3L antibody, PC3-96 antibody, APG3-LIKE antibody

Database links

Human UniProt/Swiss-Prot:Q9NT62

Protein names

APG3, APG3L, PC3-96, APG3-LIKE

Protein function

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Protein tissue specificity

Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.

Protein sequence and domain

Belongs to the ATG3 family.

Protein post-translational modifications

Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.; Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.

Protein cellular localization

Cytoplasm .

Background

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Research area

All research areas>Synthesis and Degradation>Atg
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize ATG3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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