Rabbit polyclonal ATG4D antibody (A7842)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ATG4D antibody (A7842)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal ATG4D antibody.

Alternative names:

Check alternative names for the antibody

Expand

APG4D antibody, AUTL4 antibody, APG4-D antibody
9830134P12Rik antibody|Cysteine protease ATG4D antibody|Cysteine protease involved in autophagy antibody|MGC31226 antibody|Anti-ATG4D antibody (ab137621)

SCBT cat No: sc-138835|sc-138837|

Name

Rabbit polyclonal ATG4D antibody

Catalogue No.

A7842

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ATG4D

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 53kDa/Observed: Refer to figures

Formulation

ATG4D antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ATG4D antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

APG4D antibody, AUTL4 antibody, APG4-D antibody

Database links

Human UniProt/Swiss-Prot:Q86TL0

Protein names

APG4D, AUTL4, APG4-D

Protein function

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.; Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.

Protein tissue specificity

Mainly expressed in skeletal muscle and, to a lower extent, in testis.

Protein sequence and domain

Residues 64 to 103 constitute a cryptic mitochondrial transit peptide which is revealed after cleavage by caspase of residues 1 to 63 upon oxidative stress and cell death. It acts then as a functional transit peptide, and allows the import of the cleaved protein into the mitochondria .Belongs to the peptidase C54 family.

Protein post-translational modifications

Cleaved by CASP3 during apoptosis which leads to increased activity. The cleavage by CASP3 reveals a cryptic mitochondrial targeting sequence immediately downstream of their canonical caspase cleavage sites which leads to mitochondroal import of the protein.

Protein cellular localization

Cysteine protease ATG4D: Cytoplasm .; Cysteine protease ATG4D, mitochondrial: Cytoplasm . Mitochondrion matrix . Note: Imported into mitochondrial matrix after cleavage by CASP3 during oxidative stress and cell death.

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.

Research area

All research areas>Synthesis and Degradation>Atg
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize ATG4D antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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