Rabbit polyclonal ATG5 antibody (A0203)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ATG5 antibody (A0203)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ATG5 antibody,APG5 antibody,APG5-LIKE antibody,APG5L antibody,ASP antibody,hAPG5 antibody
APG 5 antibody|ATG5_HUMAN antibody|Autophagy protein 5 antibody|hAPG5 antibody|Homolog of S Cerevisiae autophagy 5 antibody|OTTHUMP00000040507 antibody|Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327)

SCBT cat No: sc-133158|sc-8667|sc-515347|sc-33210|sc-8666|

Name

Rabbit polyclonal ATG5 antibody

Catalogue No.

A0203

Reactivity

Human

Immunogen

Recombinant protein of human ATG5

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 32kDa/Observed: Refer to Figures

Formulation

ATG5 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ATG5 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ATG5 antibody,APG5 antibody,APG5-LIKE antibody,APG5L antibody,ASP antibody,hAPG5 antibody

Database links

Human UniProt/Swiss-Prot:Q9H1Y0

Protein names

ATG5,APG5,APG5-LIKE,APG5L,ASP,hAPG5

Protein function

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.; May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.

Protein tissue specificity

Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Protein sequence and domain

Belongs to the ATG5 family.

Protein post-translational modifications

Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.; Acetylated by EP300.

Protein cellular localization

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note: Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Note

AntibodyPlus can customize ATG5 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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