Rabbit polyclonal ATG7 antibody (A0691)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal ATG7 antibody (A0691)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ATG7 antibody,APG7-LIKE antibody,APG7L antibody,DKFZp434N0735 antibody,GSA7 antibody
1810013K23Rik antibody|DKFZp434N0735 antibody|GSA 7 antibody|GSA7 antibody|hAGP7 antibody|Ubiquitin activating enzyme E1 like protein antibody|Ubiquitin-activating enzyme E1-like protein antibody|Ubiquitin-like modifier-activating enzyme ATG7 antibody|Anti-Apg7 antibody [EP1759Y] (ab52472)

SCBT cat No: sc-376212|sc-33211|sc-12497|sc-8668|

Name

Rabbit polyclonal ATG7 antibody

Catalogue No.

A0691

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ATG7

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 78kDa/Observed: Refer to Figures

Formulation

ATG7 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

ATG7 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ATG7 antibody,APG7-LIKE antibody,APG7L antibody,DKFZp434N0735 antibody,GSA7 antibody

Database links

Human UniProt/Swiss-Prot:O95352

Protein names

ATG7,APG7-LIKE,APG7L,DKFZp434N0735,GSA7

Protein function

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.

Protein tissue specificity

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Protein sequence and domain

The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.; The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.Belongs to the ATG7 family.

Protein post-translational modifications

Acetylated by EP300.

Protein cellular localization

Cytoplasm . Preautophagosomal structure . Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Note

AntibodyPlus can customize ATG7 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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