Rabbit polyclonal AURKA antibody (A2121)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal AURKA antibody (A2121)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

AIK antibody, ARK1 antibody, AURA antibody, AURORA2 antibody, BTAK antibody, MGC34538 antibody, STK15 antibody, STK6 antibody, STK7 antibody
AIK antibody|AYK antibody|AYK1 antibody|Breast tumor-amplified kinase antibody|BTAK antibody|IAK1 antibody|Serine/threonine-protein kinase 12 antibody|Serine/threonine-protein kinase 15 antibody|Serine/threonine-protein kinase 5 antibody|Serine/threonine-protein kinase 6 antibody|Serine/threonine-protein kinase aurora-A antibody|Serine/threonine-protein kinase aurora-B antibody|STK-1 antibody|STK1 antibody|STK12 antibody|STK15 antibody|STK5 antibody|STK6 antibody|Anti-Aurora A antibody [35C1] (ab13824)

SCBT cat No: sc-56881|sc-514374|sc-398814|sc-25425|sc-14318|sc-65987|sc-393357|

Name

Rabbit polyclonal AURKA antibody

Catalogue No.

A2121

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human AURKA

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 46kDa/Observed: Refer to Figures

Formulation

AURKA antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

AURKA antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

AIK antibody, ARK1 antibody, AURA antibody, AURORA2 antibody, BTAK antibody, MGC34538 antibody, STK15 antibody, STK6 antibody, STK7 antibody

Database links

Human UniProt/Swiss-Prot:O14965

Protein names

AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7

Protein function

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

Protein tissue specificity

Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

Protein sequence and domain

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis.; Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.

Protein cellular localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Note: Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase . Colocalized with SIRT2 at centrosome . Moves to the midbody during both telophase and cytokinesis . Associates with both the pericentriolar material (PCM) and centrioles .

Background

Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

Research area

All research areas>Kinases and Phosphatases>ARK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize AURKA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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