Rabbit polyclonal BAD antibody (A1593)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BAD antibody (A1593)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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BBC2 antibody, BCL2L8 antibody
AI325008 antibody|BAD antibody|BAD_HUMAN antibody|BBC 2 antibody|BBC2 antibody|BBC6 antibody|Bcl 2 Antagonist of Cell Death antibody|Bcl 2 Binding Component 6 antibody|BCL X / BCL 2 Binding Protein antibody|BCL X Binding Protein antibody|Bcl XL/Bcl 2 Associated Death Promoter antibody|Bcl-2-binding component 6 antibody|Bcl-2-like protein 8 antibody|Bcl-XL/Bcl-2-associated death promoter antibody|Bcl2 antagonist of cell death antibody|BCL2 antagonist of cell death protein antibody|BCL2 associated agonist of cell death antibody|Bcl2 Associated Death Promoter antibody|BCL2 binding component 6 antibody|BCL2 binding protein antibody|Bcl2 Like 8 Protein antibody|Bcl2-L-8 antibody|BCL2L8 antibody|Proapoptotic BH3 Only Protein antibody|Anti-Bad antibody [Y208] (ab32445)

SCBT cat No: sc-8044|sc-7869|sc-942|sc-166324|sc-33787|sc-32763|sc-48350|sc-137138|sc-365505|sc-365543|

Name

Rabbit polyclonal BAD antibody

Catalogue No.

A1593

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human BAD

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:10 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 18kDa/Observed: Refer to Figures

Formulation

BAD antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BAD antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BBC2 antibody, BCL2L8 antibody

Database links

Human UniProt/Swiss-Prot:Q92934

Protein names

BBC2, BCL2L8

Protein function

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Protein tissue specificity

Expressed in a wide variety of tissues.

Protein sequence and domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.Belongs to the Bcl-2 family.

Protein post-translational modifications

Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.; Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.

Protein cellular localization

Mitochondrion outer membrane. Cytoplasm . Note: Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm.

Background

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Research area

All research areas>Tumor Suppressor/Apoptosis>Bad
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize BAD antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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