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Rabbit polyclonal BAD antibody (A1593)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal BAD antibody.
Check alternative names for the antibodyExpand
BBC2 antibody, BCL2L8 antibody
AI325008 antibody|BAD antibody|BAD_HUMAN antibody|BBC 2 antibody|BBC2 antibody|BBC6 antibody|Bcl 2 Antagonist of Cell Death antibody|Bcl 2 Binding Component 6 antibody|BCL X / BCL 2 Binding Protein antibody|BCL X Binding Protein antibody|Bcl XL/Bcl 2 Associated Death Promoter antibody|Bcl-2-binding component 6 antibody|Bcl-2-like protein 8 antibody|Bcl-XL/Bcl-2-associated death promoter antibody|Bcl2 antagonist of cell death antibody|BCL2 antagonist of cell death protein antibody|BCL2 associated agonist of cell death antibody|Bcl2 Associated Death Promoter antibody|BCL2 binding component 6 antibody|BCL2 binding protein antibody|Bcl2 Like 8 Protein antibody|Bcl2-L-8 antibody|BCL2L8 antibody|Proapoptotic BH3 Only Protein antibody|Anti-Bad antibody [Y208] (ab32445)
SCBT cat No: sc-8044|sc-7869|sc-942|sc-166324|sc-33787|sc-32763|sc-48350|sc-137138|sc-365505|sc-365543|
Rabbit polyclonal BAD antibody
|Catalogue No.|| |
Human, Mouse, Rat
A synthetic peptide of human BAD
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 18kDa/Observed: Refer to Figures
BAD antibody was tube-contained.
BAD antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
BBC2 antibody, BCL2L8 antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
|Protein tissue specificity|| |
Expressed in a wide variety of tissues.
|Protein sequence and domain|| |
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.Belongs to the Bcl-2 family.
|Protein post-translational modifications|| |
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.; Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
|Protein cellular localization|| |
Mitochondrion outer membrane. Cytoplasm . Note: Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm.
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).
AntibodyPlus can customize BAD antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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