Rabbit polyclonal BAG1 antibody (A1104)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BAG1 antibody (A1104)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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Bag 1 antibody,BAG1 antibody,BAG1S antibody,HAP antibody,BAG1M antibody,BAG1L specific antibody,RAP46 antibody
Bag 1 antibody|BAG family molecular chaperone regulator 1 antibody|BAG-1 antibody|BAG1 antibody|BAG1_HUMAN antibody|BCL 2 Associated Athanogene 1 antibody|BCL 2 associated athanogene antibody|BCL 2 binding athanogene 1 antibody|Bcl-2-associated athanogene 1 antibody|BCL2 associated athanogene 1 antibody|BCL2 associated athanogene antibody|Glucocorticoid receptor-associated protein RAP46 antibody|HAP1 antibody|Haptoglobin antibody|RAP 46 antibody|RAP46 antibody|Anti-Bag1 antibody [Y166] (ab32109)

SCBT cat No: sc-135844|sc-56003|sc-56004|sc-377419|sc-515535|sc-939|sc-33704|sc-376848|sc-515529|sc-377454|sc-25296|sc-8348|sc-56005|

Name

Rabbit polyclonal BAG1 antibody

Catalogue No.

A1104

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human BAG1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 39kDa/Observed: Refer to Figures

Formulation

BAG1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BAG1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

Bag 1 antibody,BAG1 antibody,BAG1S antibody,HAP antibody,BAG1M antibody,BAG1L specific antibody,RAP46 antibody

Database links

Human UniProt/Swiss-Prot:Q99933

Protein names

Bag 1,BAG1,BAG1S,HAP,BAG1M,BAG1L specific,RAP46

Protein function

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.

Protein tissue specificity

Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).

Protein sequence and domain

Contains 1 BAG domain.; Contains 1 ubiquitin-like domain.

Protein post-translational modifications

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Protein cellular localization

Isoform 1: Nucleus. Cytoplasm. Note: Isoform 1 localizes predominantly to the nucleus.; Isoform 2: Cytoplasm. Nucleus. Note: Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock.; Isoform 4: Cytoplasm. Nucleus. Note: Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.

Background

Bag1 belongs to the Bcl-2 associated athanogene (BAG) family of multifunctional proteins and was the first of six related proteins isolated from humans (1,2). This widely expressed protein interacts with a number of signaling molecules (including Bcl2, HGF receptor and Raf1) as it regulates signaling molecules in pathways involving cell survival, growth and differentiation. The most common role played by Bag1 protein is as an inhibitor of proteins favoring apoptosis (2-4). Bag1 also plays a role in Raf1 signaling and binds DNA as a transcription activator (4). Bag1 protein is a well-characterized inhibitor of its binding partner HSP70 (5). A conserved carboxy-terminal BAG domain within Bag1 interacts with the ATPase domain of HSP70 to negatively regulate heat shock protein chaperone activity (6,7). The multiple isoforms of Bag1 protein generated from a single transcript share a common ubiquitin homology domain and a carboxy-terminal Hsp70 binding region but differ in length and cellular localization. The 50 kDa long (Bag1-L) isoform also contains a nuclear localization signal and is often found in the nucleus where it activates transcription. The 46 kDa intermediate (Bag1-M) isoform is found mainly in the cytoplasm but can also translocate to the nucleus when associated with other proteins. The shorter 29-33 kDa isoforms (Bag1-S, Bag-1) isoforms are found primarily in the cytoplasm (8). High expression of the anti-apoptotic Bag1 protein correlates with increased survival in patients with particular forms of cancer, leading researchers to study possible therapeutic roles for Bag1 protein (9)

Research area

All research areas>Tumor Suppressor/Apoptosis>Bag
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize BAG1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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