Rabbit Polyclonal Bcl-2 antibody (STJ91836)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Bcl-2 antibody (STJ91836)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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BCL2 antibody,|Apoptosis regulator Bcl 2 antibody|Apoptosis regulator Bcl-2 antibody|Apoptosis regulator Bcl2 antibody|AW986256 antibody|B cell CLL/lymphoma 2 antibody|B cell leukemia/lymphoma 2 antibody|Bcl-2 antibody|Bcl2 antibody|BCL2_HUMAN antibody|C430015F12Rik antibody|D630044D05Rik antibody|D830018M01Rik antibody|Leukemia/lymphoma, B-cell, 2 antibody|Oncogene B-cell leukemia 2 antibody|PPP1R50 antibody|Protein phosphatase 1, regulatory subunit 50 antibody|Anti-Bcl-2 antibody [E17] (ab32124)
SCBT cat No: sc-509|sc-56015|sc-23960|sc-130307|sc-578|sc-70411|sc-130308|sc-65392|sc-783|sc-7382|sc-492|sc-56018|sc-81002|sc-169990|sc-169989|sc-32741|sc-185|sc-13038|sc-70414|

Name

Bcl-2 Polyclonal Antibody

Catalogue No.

STJ91836

Reactivity

Human

Specificity

Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.

Immunogen

Synthesized peptide derived from Bcl-2 at AA range 40-120

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

26 kDa

Formulation

Bcl-2 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Bcl-2 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Apoptosis regulator Bcl-2 antibody

Database links

Human UniProt/Swiss-Prot:P10415;Mouse UniPort/Swiss-Prot: P10417;Rat UniProt/Swiss-Port: P49950;Human Entrez Gene: 596;Mouse Entrez Gene: 12043;Rat Entrez Gene: Rn.9996

Protein names

Apoptosis regulator Bcl-2

Protein function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release .

Protein tissue specificity

Expressed in a variety of tissues.

Involvement in disease

Note: A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. .

Protein sequence and domain

BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity. / The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3. / The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. / Belongs to the Bcl-2 family.

Protein post-translational modifications

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). / Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. / Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Protein cellular localization

Mitochondrion outer membrane / Single-pass membrane protein / Nucleus membrane / Single-pass membrane protein / Endoplasmic reticulum membrane / Single-pass membrane protein

Research area

All research areas>Tumor Suppressor/Apoptosis>Bcl-2
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize Bcl-2 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 54

Validation data ID: 54

Submission date: 2017-01-10

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Result figure:
Result figure

Figure description:

1. Marker, 100KD, 75KD, 50KD, 37KD, 25KD, 20KD,15KD,10KD
2-4 are Hela cells

Product name

anti-Bcl-2 antibody

Product catalog No.

STJ91836

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Poor

Antibody overall rating

Poor

Your comments

I don't get any band from this antibody. It maybe the antibody styied in the US custom for 1 week before it could be shipped to me.We asked for an replacement to test.

Detailed protocol

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Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: Bcl-2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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