Rabbit polyclonal BCL2 antibody (A2845)

$69.00$259.00

Reactivity: Human,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BCL2 antibody (A2845)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:1000 IHC 1:50 – 1:100 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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Bcl-2 antibody,PPP1R50 antibody
Apoptosis regulator Bcl 2 antibody|Apoptosis regulator Bcl-2 antibody|Apoptosis regulator Bcl2 antibody|AW986256 antibody|B cell CLL/lymphoma 2 antibody|B cell leukemia/lymphoma 2 antibody|Bcl-2 antibody|Bcl2 antibody|BCL2_HUMAN antibody|C430015F12Rik antibody|D630044D05Rik antibody|D830018M01Rik antibody|Leukemia/lymphoma, B-cell, 2 antibody|Oncogene B-cell leukemia 2 antibody|PPP1R50 antibody|Protein phosphatase 1, regulatory subunit 50 antibody|Anti-Bcl-2 antibody [E17] (ab32124)

SCBT cat No: sc-509|sc-56015|sc-23960|sc-130307|sc-578|sc-70411|sc-130308|sc-65392|sc-783|sc-7382|sc-492|sc-56018|sc-81002|sc-169990|sc-169989|sc-32741|sc-185|sc-13038|sc-70414|

Name

Rabbit polyclonal BCL2 antibody

Catalogue No.

A2845

Reactivity

Human, Rat

Immunogen

A synthetic peptide of human BCL2

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:1000
IHC 1:50 – 1:100
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 22kDa/Observed: Refer to Figures

Formulation

BCL2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BCL2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

Bcl-2 antibody,PPP1R50 antibody

Database links

Human UniProt/Swiss-Prot:P10415

Protein names

Bcl-2,PPP1R50

Protein function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release .

Protein tissue specificity

Expressed in a variety of tissues.

Protein sequence and domain

BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.; The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.; The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.Belongs to the Bcl-2 family.

Protein post-translational modifications

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Protein cellular localization

Mitochondrion outer membrane ; Single-pass membrane protein . Nucleus membrane ; Single-pass membrane protein . Endoplasmic reticulum membrane ; Single-pass membrane protein .

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Research area

All research areas>Tumor Suppressor/Apoptosis>Bcl-2
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize BCL2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


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Antibody customer reviews & validation data - 63

Validation data ID: 63

Submission date: 2017-01-18

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Result figure:
Result figure

Figure description:

1. Hela
2.Hela
3.Hela
4. Marker

Product name

Rabbit polyclonal BCL2 antibody

Product catalog No.

A2845

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Average

Antibody overall rating

Average

Your comments

There is strong incorrect size bands in the background. Use 1/500 dilution is much better than 1/1000 dilution.

Detailed protocol

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