Rabbit Polyclonal BCL2L11 antibody (A0295)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal BCL2L11 antibody (A0295)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:2000
IF 1:100 – 1:200

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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BAM antibody; BIM antibody; BOD antibody; BimL antibody; BimS antibody; BimEL antibody; BIM-beta6 antibody; BIM-beta7 antibody; BIM-alpha6 antibody; BCL2L11 antibody;
BCL2 like 11 antibody|Bcl-2-like protein 11 antibody|BCL2 interacting mediator of cell death antibody|BCL2 like 11 (apoptosis facilitator) antibody|BCL2 like protein 11 antibody|Bcl2-interacting mediator of cell death antibody|Bcl2-L-11 antibody|Bcl2l11 antibody|BIM alpha6 antibody|BIM antibody|BIM beta6 antibody|BIM beta7 antibody|BimEL antibody|BimL antibody|BOD antibody|Anti-Bim antibody [Y36] (ab32158)

SCBT cat No: sc-130511|

Name

Rabbit Polyclonal BCL2L11 antibody

Catalogue No.

A0295

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human BCL2L11

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:500 – 1:2000
IF 1:100 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

22kDa

Formulation

BCL2L11 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BAM antibody; BIM antibody; BOD antibody; BimL antibody; BimS antibody; BimEL antibody; BIM-beta6 antibody; BIM-beta7 antibody; BIM-alpha6 antibody; BCL2L11 antibody;

Database links

Human UniProt/Swiss-Prot:O43521

Protein names

BAM; BIM; BOD; BimL; BimS; BimEL; BIM-beta6; BIM-beta7; BIM-alpha6; BCL2L11;

Protein function

Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.

Protein tissue specificity

Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.

Protein sequence and domain

Belongs to the Bcl-2 family. The BH3 motif is required for interaction with Bcl-2 proteins and cytotoxicity.

Protein post-translational modifications

Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation (PubMed:15486195, PubMed:21478148). Deubiquitination catalyzed by USP27X stabilizes the protein (By similarity).; Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein.

Protein cellular localization

Endomembrane system ; Peripheral membrane protein . Note=Associated with intracytoplasmic membranes.; Isoform BimEL: Mitochondrion. Note=Translocates from microtubules to motochondria on loss of cell adherence.; Isoform BimL: Mitochondrion.; Isoform BimS: Mitochondrion.; Isoform Bim-alpha1: Mitochondrion.

Background

BCL2L11/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). BCL2L11 induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2). BCL2L11 functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which BCL2L11 expression is elevated (3-6). Three major isoforms of BCL2L11 are generated by alternative splicing: BCL2L11EL, BCL2L11L and BCL2L11S (1). The shortest form, BCL2L11S, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BCL2L11EL and BCL2L11L isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers BCL2L11 phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.

Research area

All research areas>Tumor Suppressor/Apoptosis>Bim
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize BCL2L11 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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Antibody customer reviews & validation data - 70

Validation data ID: 70

Submission date: 2017-01-18

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Result figure:
Result figure

Figure description:

1. Marker
2.Hela
3.Hela
4.Hela

Product name

Rabbit Polyclonal BCL2L11 antibody

Product catalog No.

A0295

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

There is correct size band . Good for western blot.

Detailed protocol

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