Rabbit polyclonal BCL6 antibody (A7173)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BCL6 antibody (A7173)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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BCL5 antibody, LAZ3 antibody, BCL6A antibody, ZNF51 antibody, ZBTB27 antibody
B cell CLL/lymphoma 6 antibody|B cell lymphoma 6 protein antibody|B-cell lymphoma 5 protein antibody|B-cell lymphoma 6 protein antibody|BCL 5 antibody|Bcl 6 antibody|BCL-5 antibody|BCL-6 antibody|BCL5 antibody|BCL6 antibody|BCL6_HUMAN antibody|BCL6A antibody|cys his2 zinc finger transcription factor antibody|LAZ 3 antibody|LAZ 3 protein antibody|LAZ3 antibody|Lymphoma associated zinc finger gene on chromosome 3 antibody|Protein LAZ-3 antibody|ZBTB 27 antibody|ZBTB27 antibody|Zinc finger and BTB domain containing protein 27 antibody|Zinc finger and BTB domain-containing protein 27 antibody|Zinc finger protein 51 antibody|zinc finger transcription factor BCL6S antibody|ZNF 51 antibody|ZNF51 antibody|Anti-Bcl6 antibody (ab19011)

SCBT cat No: sc-70414|sc-130916|sc-368|sc-7388|sc-365618|sc-858|sc-56625|

Name

Rabbit polyclonal BCL6 antibody

Catalogue No.

A7173

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human BCL6

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 79kDa/Observed: Refer to figures

Formulation

BCL6 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BCL6 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BCL5 antibody, LAZ3 antibody, BCL6A antibody, ZNF51 antibody, ZBTB27 antibody

Database links

Human UniProt/Swiss-Prot:P41182

Protein names

BCL5, LAZ3, BCL6A, ZNF51, ZBTB27

Protein function

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5′-TTCCTAGAA-3′ (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Protein tissue specificity

Expressed in germinal center T- and B-cells and in primary immature dendritic cells.

Protein sequence and domain

The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2 . Interaction with corepressors through the BTB domain is needed to facilitate the rapid proliferation and survival of GC B-cells but is not involved in the T(FH) formation and BCL6-mediated suppression of T(H)2 and T(H)17 differentiationrequired for GC formation (By similarity).Contains 1 BTB (POZ) domain.; Contains 6 C2H2-type zinc fingers.

Protein post-translational modifications

Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-333 and Ser-343. Phosphorylated by ATM in response to genotoxic stress. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.; Polyubiquitinated. Polyubiquitinated by SCF(FBXO11), leading to its degradation by the proteasome.; Acetylated at Lys-379 by EP300 which inhibits the interaction with NuRD complex and the transcriptional repressor function. Deacetylated by HDAC- and SIR2-dependent pathways.

Protein cellular localization

Nucleus .

Background

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Research area

All research areas>Transcription Regulators>Bcl-6
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize BCL6 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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