Rabbit polyclonal BID antibody (A0210)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BID antibody (A0210)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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BID antibody,FP497 antibody,MGC15319 antibody,MGC42355 antibody
Apoptic death agonist antibody|Apoptotic death agonist BID antibody|BH3 interacting domain death agonist antibody|BH3 interacting domain death agonist p11 antibody|BH3 interacting domain death agonist p13 antibody|BH3 interacting domain death agonist p15 antibody|BH3-interacting domain death agonist p11 antibody|BID antibody|BID isoform ES(1b) antibody|BID isoform L(2) antibody|BID isoform Si6 antibody|BID_HUMAN antibody|Desmocollin type 4 antibody|FP497 antibody|Human BID coding sequence antibody|MGC15319 antibody|MGC42355 antibody|p11 BID antibody|p13 BID antibody|p15 BID antibody|p22 BID antibody|Anti-Bid antibody [Y8] (ab32060)

SCBT cat No: sc-56025|sc-373939|sc-6538|sc-6291|sc-514622|sc-11423|sc-6539|sc-376866|

Name

Rabbit polyclonal BID antibody

Catalogue No.

A0210

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human BID

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 22kDa/Observed: Refer to Figures

Formulation

BID antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BID antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BID antibody,FP497 antibody,MGC15319 antibody,MGC42355 antibody

Database links

Human UniProt/Swiss-Prot:P55957

Protein names

BID,FP497,MGC15319,MGC42355

Protein function

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Protein tissue specificity

Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).

Protein sequence and domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

Protein post-translational modifications

TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.; p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

Protein cellular localization

Cytoplasm . Mitochondrion membrane . Note: When uncleaved, it is predominantly cytoplasmic.; BH3-interacting domain death agonist p15: Mitochondrion membrane . Note: Translocates to mitochondria as an integral membrane protein.; BH3-interacting domain death agonist p13: Mitochondrion membrane . Note: Associated with the mitochondrial membrane.; Isoform 1: Cytoplasm.; Isoform 3: Cytoplasm.; Isoform 2: Mitochondrion membrane. Note: A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.

Background

The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.

Research area

All research areas>Tumor Suppressor/Apoptosis>BID
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize BID antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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Antibody customer reviews & validation data - 71

Validation data ID: 71

Submission date: 2017-01-18

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Result figure:
Result figure

Figure description:

1. Hela
2.Hela
3.Hela
4. Marker

Product name

Rabbit polyclonal BID antibody

Product catalog No.

A0210

Product type

Primary antibody

Supplier

ABclonal

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

very good image in western blot. It is correct size.

Detailed protocol

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