Rabbit polyclonal BIRC5 antibody (A1551)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal BIRC5 antibody (A1551)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

API4 antibody, EPR-1 antibody
API4 antibody|Apoptosis inhibitor 4 antibody|Apoptosis inhibitor survivin antibody|Apoptosis inhibitor4 antibody|Baculoviral IAP repeat containing 5 antibody|Baculoviral IAP repeat containing protein 5 antibody|Baculoviral IAP repeat-containing protein 5 antibody|BIRC 5 antibody|BIRC5 antibody|BIRC5_HUMAN antibody|EPR 1 antibody|IAP4 antibody|Survivin variant 3 alpha antibody|SVV antibody|TIAP antibody|Anti-Survivin antibody [EP2880Y] (ab76424)

SCBT cat No: sc-23758|sc-325227|sc-12913|sc-16852|sc-130606|sc-30037|sc-18205|sc-130219|sc-248320|sc-71794|sc-32275|sc-32276|sc-12413|sc-101812|

Name

Rabbit polyclonal BIRC5 antibody

Catalogue No.

A1551

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Protein of human BIRC5

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 16kDa/Observed: Refer to Figures

Formulation

BIRC5 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

BIRC5 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

API4 antibody, EPR-1 antibody

Database links

Human UniProt/Swiss-Prot:O15392

Protein names

API4, EPR-1

Protein function

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Protein tissue specificity

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Protein sequence and domain

The BIR repeat is necessary and sufficient for LAMTOR5 binding.Belongs to the IAP family.; Contains 1 BIR repeat.

Protein post-translational modifications

Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.; In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes . Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities . Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity .; Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

Protein cellular localization

Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.

Background

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Research area

All research areas>Tumor Suppressor/Apoptosis>Survivin
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize BIRC5 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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